PP1 (Protein Phosphatase 1), also known as PPP1, is an enzyme that removes phosphate groups from serine or threonine residues. The holoenzyme is composed of two subunits. The catalytic subunit is highly conserved throughout evolution, and interacts with a wide range of proteins acting as regulatory subunits that target the enzyme to specific subcellular compartments and substrates. The catalytic subunit is ubiquitously expressed in tissues and cell lines.
PP4/PPP4C Antibody (S05-4F9)
Novus Biologicals | Catalog # NBP3-19509
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S05-4F9 expressed in HEK293
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Product Specifications
Immunogen
Recombinant protein of human PP4/PPP4C (Uniprot # P60510)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PP4/PPP4C Antibody (S05-4F9)
Western Blot: PP4/PPP4C Antibody (S05-4F9) [NBP3-19509]
Western Blot: PP4/PPP4C Antibody (S05-4F9) [NBP3-19509] - Western blot detection of PP4/PPP4C in K562, C6, 3T3, Hela cell lysates using NBP3-19509 (1:1000 diluted). Predicted band size: 35kDa. Observed band size: 35kDa.Applications for PP4/PPP4C Antibody (S05-4F9)
Application
Recommended Usage
Western Blot
1/1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PP4
Long Name
Protein Phosphatase 4
Alternate Names
PP4C, PPH3, PPP4C, PPX
Gene Symbol
PPP4C
Additional PP4 Products
Product Documents for PP4/PPP4C Antibody (S05-4F9)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PP4/PPP4C Antibody (S05-4F9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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