PP1 (Protein Phosphatase 1), also known as PPP1, is an enzyme that removes phosphate groups from serine or threonine residues. The holoenzyme is composed of two subunits. The catalytic subunit is highly conserved throughout evolution, and interacts with a wide range of proteins acting as regulatory subunits that target the enzyme to specific subcellular compartments and substrates. The catalytic subunit is ubiquitously expressed in tissues and cell lines.
PP4/PPP4C Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-99380
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PP4/PPP4C.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for PP4/PPP4C Antibody - Azide and BSA Free
Western Blot: PP4/PPP4C Antibody [NBP2-99380]
Western Blot: PP4/PPP4C Antibody [NBP2-99380] - Anti-PP4/PPP4C rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDaApplications for PP4/PPP4C Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PP4
Long Name
Protein Phosphatase 4
Alternate Names
PP4C, PPH3, PPP4C, PPX
Gene Symbol
PPP4C
Additional PP4 Products
Product Documents for PP4/PPP4C Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PP4/PPP4C Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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