PRDM16/MEL1 Antibody

Novus Biologicals | Catalog # NB100-1088

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Peptide ELISA, Flow Cytometry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PRDM16/MEL1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TSESGAFHPINHL corresponding to C-Terminus according to NP_071397.2, NP_955533.1.

Localization

Nuclear

Specificity

This antibody is expected to recognise both reported isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PRDM16/MEL1 Antibody

Flow Cytometry: PRDM16/MEL1 Antibody [NB100-1088]

Flow Cytometry: PRDM16/MEL1 Antibody [NB100-1088]

Flow Cytometry: PRDM16/MEL1 Antibody [NB100-1088] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for PRDM16/MEL1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Peptide ELISA

Detection limit 1:32000
Application Notes
Use in Western blot reported in scientific literature (PMID: 23221344).

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PRDM16/MEL1

The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36,q21)-positive MDS/AML. The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal PR domain. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Long Name

PR Domain Containing 16

Alternate Names

MDS1/EVI1-like gene 1, MEL1, PFM13

Entrez Gene IDs

63976 (Human)

Gene Symbol

PRDM16

UniProt

Q9HAZ2 (Human)

Additional PRDM16/MEL1 Products

Product Documents for PRDM16/MEL1 Antibody

Certificate of Analysis

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Product Specific Notices for PRDM16/MEL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PRDM16/MEL1 Antibody

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Protocols

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