Recombinant Protein L Type 1 Protein

Novus Biologicals | Catalog # NBP2-34963

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Bioactivity, SDS-PAGE
View all Protein L Type 1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 366 amino acids corresponding to Protein L Type 1. However it migrates with an apparent molecular mass of 46 kDa in SDS-PAGE Source: E. coli

Uniprot ID: D6S9W1

Amino Acid Sequence: CKEETPETPE TDSEEEVTIK ANLIFANGST QTAEFKGTFE KATSEAYAYA DTLKKDNGEY TVDVADKGYT LNIKFAGKEK TPEEPKEEVT IKANLIYADG KTQTAEFKGT FEEATAEAYR YADALKKDNG EYTVDVADKG YTLNIKFAGK EKTPEEPKEE VTIKANLIYA DGKTQTAEFK GTFEEATAEA YRYADLLAKE NGKYTVDVAD KGYTLNIKFA GKEKTPEEPK EEVTIKANLI YADGKTQTAE FKGTFAEATA EAYRYADLLA KENGKYTADL EDGGYTINIR FAGKKVDEKP EEKEQVTIKE NIYFEDGTVQ TATFKGTFAE ATAEAYRYAD LLSKEHGKYT ADLEDGGYTI NIRFAG

Purity

>95%, by SDS-PAGE

Endotoxin Level

Less than 0.1 EU/ug of Protein L Type 1 as determined by LAL method.

Predicted Molecular Mass

40.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Protein L is a genetically engineered protein containing 5 IgG-binding regions of protein L. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein L to ensure the maximum specific IgG binding. The recombinant Protein L is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein L binds to human, mouse, rat and pig IgG.

Specificity

Protein L binds to human, mouse, rat and pig IgG.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP2-34963
Formulation Lyophilized with no additive.
Preservative No Preservative
Concentration Lyoph
Reconstitution Dissolve in distilled water or saline.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protein L Type 1

Recombinant Protein L contains 5 kappa-binding domain. Protein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins.

Additional Protein L Type 1 Products

Product Documents for Recombinant Protein L Type 1 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Protein L Type 1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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