PSF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32156

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PSF1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 146 to 196 of human GINS Complex Subunit 1 using the numbering given in entry NP_066545.3 (GeneID 9837).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSF1 Antibody - BSA Free

Western Blot: PSF1 Antibody [NBP2-32156]

Western Blot: PSF1 Antibody [NBP2-32156]

Western Blot: PSF1 Antibody [NBP2-32156] - Detection of Human PSF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PSF1 antibody NBP2-32156 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: PSF1 Antibody [NBP2-32156]

Western Blot: PSF1 Antibody [NBP2-32156]

Western Blot: PSF1 Antibody [NBP2-32156] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PSF1 antibody NBP2-32156 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PSF1 Antibody [NBP2-32156]

Immunoprecipitation: PSF1 Antibody [NBP2-32156]

Immunoprecipitation: PSF1 Antibody [NBP2-32156] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PSF1 antibody NBP2-32156 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated PSF1, NBP2-32156 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PSF1 Antibody [NBP2-32156]

Immunoprecipitation: PSF1 Antibody [NBP2-32156]

Immunoprecipitation: PSF1 Antibody [NBP2-32156] - Detection of Human PSF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PSF1 antibody NBP2-32156 used for IP at 3 ug per reaction. PSF1 was also immunoprecipitated by rabbit anti-PSF1 antibody NBP2-30365. For blotting immunoprecipitated PSF1, NBP2-32156 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PSF1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 mcg/mg lysate

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PSF1

The yeast heterotetrameric GINS complex is made up of Sld5 (GINS4; MIM 610611), Psf1, Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]).[supplied by OMIM]

Alternate Names

GINS complex subunit 1, GINS complex subunit 1 (Psf1 homolog), KIAA0186PSF1DNA replication complex GINS protein PSF1, partner of sld five-1

Gene Symbol

GINS1

UniProt

NP_066545.3 (Human)

Additional PSF1 Products

Product Documents for PSF1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PSF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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