Rad21 Antibody (CM110-2C10) [FITC]

Novus Biologicals | Catalog # NB100-386F

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CM110-2C10
View all Rad21 Primary Antibodies »

Product Specifications

Immunogen

An N-terminal fusion protein of the C-terminal fragment of Scc1 subunit human cohesin (amino acids 544-631 of hRad21).

Reactivity Notes

Human and mouse.

Localization

Nuclear and cytoplasm

Specificity

NB100-386 is specific for both the nuclear hRad21 protein and the cleaved cytoplasmic hRad21 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Rad21 Antibody (CM110-2C10) [FITC]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Rad21 (CM110-2C10) antibody can be used in Western blot, where two bands are seen. The full-length Rad21 is seen around 130 kDa, while the cleaved Rad21 is seen around 70 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Rad21

The cohesin complex plays an important role in tethering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys the cohesin complex by means of cleavaging its Scc1 subunit (Mcd1/Scc1/hRad21), allowing the chromatids to separate and divide with the cell. During apoptosis the C-terminal portion of hRad21 is cleaved and translocated to the cytoplasm. This cleavage and translocation may act as a nuclear signal that promotes and accelerates subsequent events of apoptosis (2).

Alternate Names

double-strand-break repair protein rad21 homolog, hHR21FLJ25655, HR21, KIAA0078RAD21 (S. pombe) homolog, MCD1, Nuclear matrix protein 1, NXP-1, NXP1HRAD21, protein involved in DNA double-strand break repair, RAD21 homolog (S. pombe), SCC1 homolog, SCC1FLJ40596

Gene Symbol

RAD21

Additional Rad21 Products

Product Documents for Rad21 Antibody (CM110-2C10) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rad21 Antibody (CM110-2C10) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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