Rad21 Antibody (CM110-2C10) - BSA Free

Novus Biologicals | Catalog # NB100-386

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CM110-2C10

Format

BSA Free
View all Rad21 Primary Antibodies »

Product Specifications

Immunogen

An N-terminal fusion protein of the C-terminal fragment of Scc1 subunit human cohesin (amino acids 544-631 of hRad21).

Localization

Nuclear and cytoplasm

Specificity

NB100-386 is specific for both the nuclear hRad21 protein and the cleaved cytoplasmic hRad21 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Rad21 Antibody (CM110-2C10) - BSA Free

Western Blot: Rad21 Antibody (CM110-2C10)BSA Free [NB100-386]

Western Blot: Rad21 Antibody (CM110-2C10)BSA Free [NB100-386]

Western Blot: Rad21 Antibody (CM110-2C10) [NB100-386] - Western blot with monoclonal anti-Rad21 (NB100-386) in HEK293 lysates.
Simple Western: Rad21 Antibody (CM110-2C10)BSA Free [NB100-386]

Simple Western: Rad21 Antibody (CM110-2C10)BSA Free [NB100-386]

Simple Western: Rad21 Antibody (CM110-2C10) [NB100-386] - Simple Western lane view shows a specific band for Rad21 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for Rad21 Antibody (CM110-2C10) - BSA Free

Application
Recommended Usage

Simple Western

1:50

Western Blot

1:500 (ECL)
Application Notes
This Rad21 (CM110-2C10) antibody can be used in Western blot, where two bands are seen. The full-length Rad21 is seen around 130 kDa, while the cleaved Rad21 is seen around 70 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Hek293 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 72 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rad21

The cohesin complex plays an important role in tethering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys the cohesin complex by means of cleavaging its Scc1 subunit (Mcd1/Scc1/hRad21), allowing the chromatids to separate and divide with the cell. During apoptosis the C-terminal portion of hRad21 is cleaved and translocated to the cytoplasm. This cleavage and translocation may act as a nuclear signal that promotes and accelerates subsequent events of apoptosis (2).

Alternate Names

double-strand-break repair protein rad21 homolog, hHR21FLJ25655, HR21, KIAA0078RAD21 (S. pombe) homolog, MCD1, Nuclear matrix protein 1, NXP-1, NXP1HRAD21, protein involved in DNA double-strand break repair, RAD21 homolog (S. pombe), SCC1 homolog, SCC1FLJ40596

Entrez Gene IDs

5885 (Human); 19357 (Mouse)

Gene Symbol

RAD21

UniProt

O60216 (Human)

Additional Rad21 Products

Product Documents for Rad21 Antibody (CM110-2C10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rad21 Antibody (CM110-2C10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rad21 Antibody (CM110-2C10) - BSA Free

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Protocols

View specific protocols for Rad21 Antibody (CM110-2C10) - BSA Free (NB100-386):

Rad21 Antibody (CM110-2C10):
1. Load protein on gel (ie: ~8 ug of non-transfected, control HeLa extract) and run.
2. Transfer protein to nitrocellulose (Schleicher&Schuell, cat# BA83).
3. Block the membrane with 5% milk for 30 minutes at room temperature.
4. Incubate the membrane with anti-cohesin [cat# NB 100-386] (1:500), overnight at 4C or 2 h at room temperature. Milk or PBS-T are good for this dilution.
5. Wash the membrane 3 times, 10 minutes per wash in PBS with 0.1% Tween-20 (PBS-T).
6. Incubate with secondary antibody for 30 minutes at room temperature. Use milk in PBS-T as a diluent.
7. Rinse the membrane 2 times with deionized water and place in an ECL working solution.
8. Expose to the film.

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FAQs

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