Rat CCR6 Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB8320V-100UG
Detection of CCR6 in HEK293 Human Cell Line Transfected with Rat CCR6 and eGFP by Flow Cytometry.
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Rat CCR6 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat CCR6 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 876515
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NSO mouse myeloma cell line transfected with rat CCR6
Met1-Met366
Accession # Q5BK58
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CCR6 in HEK293 Human Cell Line Transfected with Rat CCR6 and eGFP by Flow Cytometry. View Larger

Detection of CCR6 in HEK293 Human Cell Line Transfected with Rat CCR6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) rat CCR6 or (B) irrelevant transfectant and eGFP was stained with Mouse Anti-Rat CCR6 Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB8320V). Quadrant markers were set based on control antibody staining (Catalog # IC0041V). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR6

CCR6 is an approximately 45 kDa G-protein linked seven transmembrane domain receptor that binds the chemokine CCL20/MIP-3 alpha. Among hematopoietic cells, CCR6 is expressed on B cells, immature dendritic cells, neutrophils, and various T cell subsets (e.g. CD4+ T cells, regulatory T cells, effector memory Th17 cells, and g/d T cells). It is upregulated in several cancers, Alzheimer’s brain, and the airways in obstructive pulmonary disease. CCR6 plays an important role in mucosal immunity, IgE mediated allergic reactions, immune cell infiltration into sites of inflammation, and tumor cell invasiveness. It also promotes chemotaxis and tumor angiogenesis in response to beta defensins. Rat CCR6 shares 74% and 87% amino acid sequence identity with human and mouse CCR6, respectively.

Entrez Gene IDs
1235 (Human); 12458 (Mouse); 308163 (Rat)
Alternate Names
BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22

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