Rat CCR6 Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat CCR6 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 876515
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NSO mouse myeloma cell line transfected with rat CCR6
    Met1-Met366
    Accession # Q5BK58
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR6 in HEK293 Human Cell Line Transfected with Rat CCR6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with rat CCR6 and eGFP was stained with Mouse Anti-Rat CCR6 Monoclonal Antibody (Catalog # MAB8320) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR6

CCR6 is an approximately 45 kDa G-protein linked seven transmembrane domain receptor that binds the chemokine CCL20/MIP-3 alpha. Among hematopoietic cells, CCR6 is expressed on B cells, immature dendritic cells, neutrophils, and various T cell subsets (e.g. CD4+ T cells, regulatory T cells, effector memory Th17 cells, and g/d T cells). It is upregulated in several cancers, Alzheimer’s brain, and the airways in obstructive pulmonary disease. CCR6 plays an important role in mucosal immunity, IgE mediated allergic reactions, immune cell infiltration into sites of inflammation, and tumor cell invasiveness. It also promotes chemotaxis and tumor angiogenesis in response to beta defensins. Rat CCR6 shares 74% and 87% amino acid sequence identity with human and mouse CCR6, respectively.

  • Entrez Gene IDs:
    1235 (Human); 12458 (Mouse); 308163 (Rat)
  • Alternate Names:
    BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 22  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 5
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF007 16  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 8  
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Donkey Anti-Mouse IgG NL557 Affinity Purified PAb

Flow, IHC, ICC NL007 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 4
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 6
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF018 2  
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Mouse IgG Antibody

WB AF007 3
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 2  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 2
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 1
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018
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