rE. coli Fpg with Buffer
rE. coli Fpg with Buffer Summary
E. coli Formamidopyrimidine-DNA Glycosylase (Fpg) releases damaged bases preferentially from duplex DNA. It has an associated class I AP lyase activity, leaving both 3' and 5' phosphoryl groups. This results from a beta -elimination reaction at the AP sites, producing a single nucleotide gap in the DNA. The enzyme has a molecular weight of 30.2 kDa. One Unit cleaves 1 pmole of a labeled oligonucleotide probe containing 8-oxoguanine, within an oligonucleotide duplex in one hour at 37 °C.
• 10X REC™ Buffer 10
• E. coli Fpg
For research use only. Not for diagnositic use.
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