Recombinant Cotton Rat CD8 alpha Protein, CF

R&D Systems | Catalog # 9648-CD

R&D Systems
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Key Product Details

  • R&D Systems NS0-derived Recombinant Cotton Rat CD8 alpha Protein (9648-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

AAL55392
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Product Specifications

Source

Mouse myeloma cell line, NS0-derived cotton rat CD8 protein
Ser24-Asp182, with a C-terninal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser24

Predicted Molecular Mass

18 kDa

SDS-PAGE

26-32 kDa, reducing conditions

Activity

Bioassay data are not available.

Reviewed Applications

Read 1 review rated 5 using 9648-CD in the following applications:

Formulation, Preparation, and Storage

9648-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD8

CD8 is a dimeric complex made up of two Ig superfamily members (1). The CD8 alpha chain is a type I transmembrane glycoprotein that can dimerize either with itself forming CD8 alpha alpha, or with a 30-35 kDa CD8 beta chain, forming CD8 alpha beta (2). CD8 alpha contains one V-type Ig-like domain in its extracellular region that binds to class I MHC molecules. CD8 alpha beta is a TCR co-receptor, while CD8 alpha alpha promotes T cell survival and differentiation (1, 3). The mature cotton rat CD8 alpha consists of a 159 amino acid (aa) extracellular domain (ECD), and runs at ~30 kDa under reduced condition. Within the ECD, cotton rat CD8 alpha shares 49%, 47%, and 54% amino acid sequence identity with human, mouse, and rat CD8 alpha, respectively.

References

  1. Laugel, B. et al. (2011) J. Leukoc. Biol. 90:1089.
  2. Snow, P.M. and C. Terhorst (1983) J. Biol. Chem. 258:14675.
  3. Cole, D.K. et al. (2012) Immunology 137:139.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

AAL55392 (Cotton Rat)

Additional CD8 Products

Product Documents for Recombinant Cotton Rat CD8 alpha Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Cotton Rat CD8 alpha Protein, CF

For research use only

Customer Reviews for Recombinant Cotton Rat CD8 alpha Protein, CF (1)

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  • Recombinant Cotton Rat CD8 alpha Protein, CF
    Name: Leslie Priddy
    Application: In vivo study
    Verified Customer | Posted 04/20/2018

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