Recombinant Cynomolgus ALCAM/CD166 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10125-AL-100
Recombinant Cynomolgus ALCAM/CD166 Fc Chimera Protein, CF SDS-PAGE
2 Images
Product Details
FAQs
Reviews

Recombinant Cynomolgus ALCAM/CD166 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Cynomolgus Monkey ALCAM/CD166 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CD6 Fc Chimera binds with an ED50 of 10-60 ng/mL
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey ALCAM/CD166 protein
Cynomolgus Monkey ALCAM/CD166
(Trp28-Ala526)
Accession # XP_005548303
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Trp28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
82 kDa
SDS-PAGE
90-125 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10125-AL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey ALCAM/CD166Fc Chimera (Catalog # 10125-AL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 90-125 kDa and 180-250 kDa, respectively.

Binding Activity View Larger

When Recombinant Cynomolgus Monkey ALCAM/CD166 Fc Chimera (Catalog # 10125-AL) is coated onto a microplate at 1 µg/mL, Biotinylated Recombinant Human CD6 Fc Chimera binds with an ED50of 10-60 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: ALCAM/CD166

ALCAM (activated leukocyte cell adhesion molecule), designated CD166, is a 100‑110 kDa type I transmembrane glycoprotein and a member of the Ig CAM family within the immunoglobulin superfamily (1). The cynomologous ALCAM amino acid sequence includes a signal peptide, an extracellular domain (ECD) with two V‑type and three C2‑type Ig‑like domains, a transmembrane domain and a short cytoplasmic domain (1). Human ALCAM has several isoforms, including an isoform lacking most of the cytoplasmic domain and a secreted isoform (sALCAM) which antagonizes full‑length ALCAM (2, 3). Mature cynomologous ALCAM ECD shares 93% and 96% amino acid sequence identity with human and mouse/rat ALCAM, respectively. ALCAM is expressed on multiple cell types including thymic epithelium, microvascular endothelium, activated lymphocytes and monocytes, and monocyte‑derived dendritic cells (1, 4). ALCAM mediates low‑affinity adhesion with itself or the cysteine‑rich scavenger receptor CD6 to regulate T cell development, immunological synapses (IS), and cell migration through endothelial junctions (1‑11). ALCAM on thymic epithelia mediates adhesion to CD6 on CD4+CD8+ T cells (6). Adhesion of ALCAM‑expressing antigen presenting cells and CD6‑expressing T cells stabilizes the early IS, while later it enhances CD3 effects on T cell proliferation, CD25 expression, and Th1 commitment (4, 7, 8). High ALCAM expression at the blood‑brain barrier in active multiple sclerosis, and its mouse model (EAE), promotes leukocyte migration to the brain (8, 9). High ALCAM expression on melanoma cell lines appears to be pro‑metastatic, but anti‑metastatic activity has been reported in breast cancer (3, 10, 11). ALCAM may influence expression or adhesion of the neuronal adhesion molecule NCAM‑L1, both in the developing retina and invasive melanoma (2, 12).

References
  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. van Kilsdonk, J.W.J. et al. (2008) Cancer Res. 68:3671.
  3. Ikeda, K. and T. Quertermous (2004) J. Biol. Chem. 279:55315.
  4. Zimmerman, A.W. et al. (2006) Blood 107:3212.
  5. van Kempen, L.C. et al. (2001) J. Biol. Chem. 276:25783.
  6. Castro, M.A.A. et al. (2007) J. Immunol. 178:4351.
  7. Nair, P. et al. (2010) Clin. Exp. Immunol. 162:116.
  8. Masedunskas, A. et al. (2006) FEBS Lett. 580:2637.
  9. Cayrol, R. et al. (2008) Nat. Immunol. 9:137.
  10. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
Long Name
Activated Leukocyte Cell Adhesion Molecule
Entrez Gene IDs
214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)
Alternate Names
activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Cynomolgus ALCAM/CD166 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Cynomolgus ALCAM/CD166 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Cynomolgus ALCAM/CD166 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review