Recombinant Human BTN1A1/Butyrophilin Protein, CF

    
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  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-2.5 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived human BTN1A1/Butyrophilin protein
    Ala27-Arg242 with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala27
  • Structure / Form
    Non-covalent dimer
  • Predicted Molecular Mass
    25 kDa
  • SDS-PAGE
    33-41 kDa, reducing conditions
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8467-BT
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 100 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Images
1 μg/lane of Recombinant Human BTN1A1/Butyrophilin was resolved with
SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 37.2 kDa and 34.9 kDa, respectively.
Recombinant Human BTN1A1/Butyrophilin (Catalog # 8467-BT) inhibits anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.The ED50 for this effect is 0.5-2.5 μg/mL.
Background: BTN1A1/Butyrophilin

Butyrophilin 1A1 (also called BTN1A1), a 55 kDa type I transmembrane glycoprotein, is a member of the Ig superfamily. BTN1A1 is 494 amino acids (aa) long and is composed of an extracellular domain (ECD) (aa 27-242), a transmembrane domain and a cytoplasmic tail (aa 270-526) which contains the B30.2 domain. The BTN1A1 ECD displays two predicted IgV and IgC domains as do B7 and Skint proteins which interact with other Ig superfamily members (1).The B30.2 domain of BTN1A1 binds to xanthine oxidoreductase (XOR) (2). This interaction stabilizes the association of XOR with the milk fat globule membrane and appears to be essential in the control of milk fat globule secretion (3, 4, 5). Binding to XOR is conserved among BTN1A1 orthologs, but is not shared by BTN2A1 or BTN3A1 (2). The B30.2 domain of butyrophilins is also described as a sensor for detecting changes in intracellular phopho-antigen (pAg) concentrations. B30.2 binding to pAg induces a cascade of events leading to the activation of gamma δ T cells (6). In vitro, BTN1A1 has an inhibitory effect on CD4+ T cell proliferation, and in addition reduces expression of cytokines associated with T cell activation such as IL-2 and IFN-gamma (7, 8). Furthermore, in vivo, BTN1A1 has a protective effect against the development of experimental autoimmune encephalomyelitis (EAE) (9). The ECD of human BTN1A1 shares 68% aa sequence identity with both mouse and rat BTN1A1. Because butyrophilins are structurally related to B7 proteins and are functionally implicated in immune regulation, they may represent an emerging family of co-stimulatory/inhibitory molecules.

  • References:
    1. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
    2. Jeong, J. et al. (2009) J. Biol. Chem. 284:22444.
    3. Vorback, C. et al. (2002) Genes Dev. 16:3223.
    4. Ogg, S.L. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10084.
    5. Robenek, H. et al. (2006) Proc. Natl. Acad. Sci. USA 103:10385.
    6. Sandstrom, A. et al. (2014) Immunity 40:490.
    7. Arnet, H.A. and Viney, J.L. (2014) Nat.Rev.Immunol.14:559.
    8. Smith, I.A. et al. (2010) J.Immunol. 184:3514.
    9. Mana, P. et al. (2004) J.Immunol. 16:489.
  • Entrez Gene IDs:
    696 (Human); 12231 (Mouse); 306956 (Rat)
  • Alternate Names:
    BTBTNbK14H9.2 (butyrophilin, subfamily 1, member A1); BTN; BTN1A1; butyrophilin subfamily 1 member A1; butyrophilin, subfamily 1, member A1
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Primary Antibodies
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His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 28  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 3  
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His Tag Antibody

WB , Flow , AP MAB050R 2  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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