Recombinant Human C1qTNF1, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human BAI3 is coated at 4 µg/mL, Recombinant Human C1qTNF1 (Catalog # 9268-TN) binds with an
    ED50 = 0.6-3.6 µg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Arg26-Pro281, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Arg26
  • Predicted Molecular Mass
    30 kDa
  • SDS-PAGE
    33-41 kDa, reducing conditions
9268-TN
 
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
WhenRecombinant Human BAI3 is coated at 4 µg/mL, Recombinant Human C1qTNF1 (Catalog #
9268-TN) binds with an ED50 of 0.6-3.6 µg/mL.
Background: C1qTNF1
C1qTNF1 (CTRP1) is an approximately 35 kDa member of the C1q family of secreted proteins and plays a role in energy metabolism and inflammation (1, 2). C1qTNF1 contains a collagen-like region and one C1q-like domain (3). Mature human C1qTNF1 shares 80% aa sequence identity with mouse and rat C1qTNF1. Circulating levels of C1qTNF1 are elevated in obesity, hypertension, and diabetes but can be decreased in the serum of diet-induced obese mice (4-6). C1qTNF1 expression is up-regulated in atherosclerotic plaques or adipose tissue by oxidized LDL or inflammatory cytokines (3, 7, 8). In turn, it induces the expression of inflammatory cytokines (7, 9) and the up-reglation of adhesion proteins on vascular endothelial cells (8). Systemically administered C1qTNF1, in contrast, can limit tissue damage following myocardial infarction (9). In skeletal muscle, C1qTNF1 promotes fatty acid oxidation, energy expenditure, insulin sensitivity, and glucose uptake and glycolysis (6, 10). It also induces the proliferation of immature chondrocytes (11) and aldosterone synthesis in the adrenal cortex (4). R&D Systems in-house testing indicates that C1qTNF1 binds to BAI3, consistent with the reported interactions between BAI3 and C1qL proteins (12).
  • References:
    1. Grebrehiwet, B. et al. (2012) Front. Immunol. 5:3.
    2. Seldin, M.M. et al. (2014) Rev. Endocr. Metab. Disord. 15:111.
    3. Kim, K.-Y. et al. (2006) FEBS Lett. 580:3953.
    4. Jeon, J.H. et al. (2008) FASEB J. 22:1502.
    5. Xin, Y. et al. (2014) Endocr. J. 61:841.
    6. Peterson, J. M. et al. (2012) J. Biol. Chem. 287:1576.
    7. Wang, X.Q. et al. (2016) Atherosclerosis 250:38.
    8. Lu, L. et al. (2016) Eur. Heart J. 37:1762.
    9. Yuasa, D. et al. (2016) FASEB J. 30:1065.
    10. Han, S. et al. (2016) J. Nutr. Biochem. 27:43.
    11. Akiyama, H. et al. (2013) Mol. Cell. Endocrinol. 369:63.
    12. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
  • Long Name:
    C1q And Tumor Necrosis Factor Related Protein 1
  • Entrez Gene IDs:
    114897 (Human); 56745 (Mouse); 303701 (Rat)
  • Alternate Names:
    C1q and tumor necrosis factor related protein 1; C1qTNF1; CTRP1; CTRP1G protein-coupled receptor-interacting protein; FLJ90694; G protein coupled receptor interacting protein; GIPcomplement C1q tumor necrosis factor-related protein 1; ZSIG37
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP MAB050 17  
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His Tag Horseradish Peroxidase-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 1  
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His Tag Biotinylated Antibody

WB BAM050 1  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999
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