Recombinant Human CNTF Protein

  (20 citations)     
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Product Details
Citations (20)
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  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.03-0.18 μg/mL.
    The proliferative effect can be enhanced up to 100-fold by the addition of Recombinant Human CNTF R alpha (Catalog # 303-CR) . Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. Davies, A.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R.A. (eds): John Willey and Sons, Ltd. 95. The ED50 for this effect is 1-3 ng/mL.
  • Source
    E. coli-derived human CNTF protein
    Ala2-Met200
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala2
  • Structure / Form

    This recombinant protein is prone to proteolytic degradation at the C-terminus, resulting in proteins with molecular masses of 22.2-22.8 kDa.

  • Predicted Molecular Mass
    22.8 kDa
  • SDS-PAGE
    23-26 kDa, reducing conditions
Product Datasheets

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Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
257-NT
 
257-NT/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose with BSA as a carrier protein.
 
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human CNTF (Catalog #
257‑NT) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.03-0.18 μg/mL.
1 μg/lane of Recombinant Human CNTF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 26 kDa.
Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.

The gene for human CNTF has been localized to the proximal region of the long arm of chromosome 11. The cDNA for human CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

  • Long Name:
    Ciliary Neurotrophic Factor
  • Entrez Gene IDs:
    1270 (Human); 25707 (Rat)
  • Alternate Names:
    ciliary neurotrophic factor; CNTF; HCNTF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Blocking p62/SQSTM1-dependent SMN degradation ameliorates Spinal Muscular Atrophy disease phenotypes
    Authors: N Rodriguez-, A Parkhitko, T Grass, RM Gibbs, EM Norabuena, N Perrimon, R Singh, LL Rubin
    J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Dissecting the Functional Consequences of De Novo DNA Methylation Dynamics in Human Motor Neuron Differentiation and Physiology
    Authors: MJ Ziller, JA Ortega, KA Quinlan, DP Santos, H Gu, EJ Martin, C Galonska, R Pop, S Maidl, A Di Pardo, M Huang, HY Meltzer, A Gnirke, CJ Heckman, A Meissner, E Kiskinis
    Cell Stem Cell, 2018;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Expression of Pluripotency Markers in Non-pluripotent Human Neural Stem and Progenitor Cells
    Authors: PH Vincent, E Benedikz, P Uhlen, O Hovatta, E Sundström
    Stem Cells Dev., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Human bone marrow harbors cells with neural crest-associated characteristics like human adipose and dermis tissues
    Authors: C Coste, V Neirinckx, A Sharma, G Agirman, B Rogister, J Foguenne, F Lallemend, A Gothot, S Wislet
    PLoS ONE, 2017;12(7):e0177962.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration
    Authors: Q Wang, Y Liu, C Zhang, F Guo, C Feng, X Li, H Shi, Z Su
    Protein Expr. Purif, 2017;133(0):152-159.
    Species: Human
    Sample Type:
  6. Generation of neuropeptidergic hypothalamic neurons from human pluripotent stem cells.
    Authors: Merkle F, Maroof A, Wataya T, Sasai Y, Studer L, Eggan K, Schier A
    Development, 2015;142(4):633-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Human iPSC-derived motoneurons harbouring TARDBP or C9ORF72 ALS mutations are dysfunctional despite maintaining viability.
    Authors: Devlin, Anna-Cla, Burr, Karen, Borooah, Shyamang, Foster, Joshua D, Cleary, Elaine M, Geti, Imbisaat, Vallier, Ludovic, Shaw, Christop, Chandran, Siddhart, Miles, Gareth B
    Nat Commun, 2015;6(0):5999.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Canine epidermal neural crest stem cells: characterization and potential as therapy candidate for a large animal model of spinal cord injury.
    Authors: Gericota B, Anderson J, Mitchell G, Borjesson D, Sturges B, Nolta J, Sieber-Blum M
    Stem Cells Transl Med, 2014;3(3):334-45.
    Species: Canine
    Sample Type: Whole Cells
    Application: Bioassay
  9. Autophagy induction enhances TDP43 turnover and survival in neuronal ALS models.
    Authors: Barmada, Sami J, Serio, Andrea, Arjun, Arpana, Bilican, Bilada, Daub, Aaron, Ando, D Michae, Tsvetkov, Andrey, Pleiss, Michael, Li, Xingli, Peisach, Daniel, Shaw, Christop, Chandran, Siddhart, Finkbeiner, Steven
    Nat Chem Biol, 2014;10(8):677-85.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Chicken embryos as a potential new model for early onset type I diabetes.
    Authors: Shi L, Ko M, Huang C, Park S, Hong M, Wu C, Ko G
    J Diabetes Res, 2014;2014(0):354094.
    Species: Chicken
    Sample Type: Whole Cells
    Application: Bioassay
  11. Involvement of ER stress in dysmyelination of Pelizaeus-Merzbacher Disease with PLP1 missense mutations shown by iPSC-derived oligodendrocytes.
    Authors: Numasawa-Kuroiwa Y, Okada Y, Shibata S, Kishi N, Akamatsu W, Shoji M, Nakanishi A, Oyama M, Osaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H
    Stem Cell Reports, 2014;2(5):648-61.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. A small molecule screen in stem-cell-derived motor neurons identifies a kinase inhibitor as a candidate therapeutic for ALS.
    Authors: Yang Y, Gupta S, Kim K, Powers B, Cerqueira A, Wainger B, Ngo H, Rosowski K, Schein P, Ackeifi C, Arvanites A, Davidow L, Woolf C, Rubin L
    Cell Stem Cell, 2013;12(6):713-26.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
    Authors: Zelinka C, Scott M, Volkov L, Fischer A
    PLoS ONE, 2012;7(9):e44477.
    Species: Chicken
    Sample Type: In Vivo
    Application: In Vivo
  14. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2012;21(3):404-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Phosphatidylinositol 3 kinase-Akt signaling serves as a circadian output in the retina.
    Authors: Ko ML, Jian K, Shi L, Ko GY
    J. Neurochem., 2009;108(6):1607-20.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: Bioassay
  16. Ciliary neurotrophic factor (CNTF) for human retinal degeneration: phase I trial of CNTF delivered by encapsulated cell intraocular implants.
    Authors: Sieving PA, Caruso RC, Tao W, Coleman HR, Thompson DJ, Fullmer KR, Bush RA
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(10):3896-901.
    Species: Human
    Sample Type: Serum
    Application: ELISA capture
  17. The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
    Authors: He W, Gong K, Smith DK, Ip NY
    FEBS Lett., 2005;579(20):4317-23.
    Species: Human
    Sample Type: Peptide
    Application: Binding Study
  18. Transitin, a nestin-related intermediate filament, is expressed by neural progenitors and can be induced in Muller glia in the chicken retina.
    Authors: Fischer AJ, Omar G
    J. Comp. Neurol., 2005;484(1):1-14.
    Species: Chicken
    Sample Type: In Vivo
    Application: In Vivo
  19. PACAP promotes sensory neuron differentiation: blockade by neurotrophic factors.
    Authors: Nielsen KM, Chaverra M, Hapner SJ, Nelson BR, Todd V, Zigmond RE, Lefcort F
    Mol. Cell. Neurosci., 2004;25(4):629-41.
    Species: Chicken
    Sample Type: Whole Cells
    Application: Bioassay
  20. Membrane distal cytokine binding domain of LIFR interacts with soluble CNTFR in vitro.
    Authors: He W, Gong K, Zhu G, Smith DK
    FEBS Lett., 2002;514(2):214-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
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Recombinant Proteins
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Recombinant Human CNTF R alpha Protein, CF

BA 303-CR/CF 1
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Recombinant Human CNTF R alpha Protein

BA 303-CR 1
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