Recombinant Human CNTF Protein

Formulations:
Catalog # Availability Size / Price Qty
257-NT-010
257-NT-050
Recombinant Human CNTF Protein Data
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Product Details
Citations (26)
FAQs
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Reviews

Recombinant Human CNTF Protein Summary

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.03-0.18 μg/mL.
The proliferative effect can be enhanced up to 100-fold by the addition of Recombinant Human CNTF R alpha (Catalog # 303-CR) .
Source
E. coli-derived human CNTF protein
Ala2-Met200
Accession #
N-terminal Sequence
Analysis
Ala2
Structure / Form

This recombinant protein is prone to proteolytic degradation at the C-terminus, resulting in proteins with molecular masses of 22.2-22.8 kDa.

Predicted Molecular Mass
22.8 kDa
SDS-PAGE
23-26 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

257-NT

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.

257-NT/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Human CNTF (Catalog # 257‑NT) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50for this effect is 0.03-0.18 μg/mL.

SDS-PAGE View Larger

1 μg/lane of Recombinant Human CNTF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 26 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.

The gene for human CNTF has been localized to the proximal region of the long arm of chromosome 11. The cDNA for human CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Long Name
Ciliary Neurotrophic Factor
Entrez Gene IDs
1270 (Human); 25707 (Rat)
Alternate Names
ciliary neurotrophic factor; CNTF; HCNTF

Citations for Recombinant Human CNTF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene
    Authors: M Chumarina, K Russ, C Azevedo, A Heuer, M Pihl, A Collin, EÅ Frostner, E Elmer, P Hyttel, G Cappellett, M Zini, S Goldwurm, L Roybon
    Acta Neuropathol Commun, 2019;7(1):208.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. V2a interneuron differentiation from mouse and human pluripotent stem cells
    Authors: JC Butts, N Iyer, N White, R Thompson, S Sakiyama-E, TC McDevitt
    Nat Protoc, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Mitigating Antagonism between Transcription and Proliferation Allows Near-Deterministic Cellular Reprogramming
    Authors: KN Babos, KE Galloway, K Kisler, M Zitting, Y Li, Y Shi, B Quintino, RH Chow, BV Zlokovic, JK Ichida
    Cell Stem Cell, 2019;25(4):486-500.e9.
    Species: Mousee
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Premature polyadenylation-mediated loss of stathmin-2 is a hallmark of TDP-43-dependent neurodegeneration
    Authors: Z Melamed, J López-Erau, MW Baughn, O Zhang, K Drenner, Y Sun, F Freyermuth, MA McMahon, MS Beccari, JW Artates, T Ohkubo, M Rodriguez, N Lin, D Wu, CF Bennett, F Rigo, S Da Cruz, J Ravits, C Lagier-Tou, DW Cleveland
    Nat. Neurosci., 2019;22(2):180-190.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. ALS-implicated protein TDP-43 sustains levels of STMN2, a mediator of motor neuron growth and repair
    Authors: JR Klim, LA Williams, F Limone, I Guerra San, BN Davis-Duse, DA Mordes, A Burberry, MJ Steinbaugh, KK Gamage, R Kirchner, R Moccia, SH Cassel, K Chen, BJ Wainger, CJ Woolf, K Eggan
    Nat. Neurosci., 2019;22(2):167-179.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Dissecting the Functional Consequences of De Novo DNA Methylation Dynamics in Human Motor Neuron Differentiation and Physiology
    Authors: MJ Ziller, JA Ortega, KA Quinlan, DP Santos, H Gu, EJ Martin, C Galonska, R Pop, S Maidl, A Di Pardo, M Huang, HY Meltzer, A Gnirke, CJ Heckman, A Meissner, E Kiskinis
    Cell Stem Cell, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Blocking p62/SQSTM1-dependent SMN degradation ameliorates Spinal Muscular Atrophy disease phenotypes
    Authors: N Rodriguez-, A Parkhitko, T Grass, RM Gibbs, EM Norabuena, N Perrimon, R Singh, LL Rubin
    J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Circadian Regulation of Mitochondrial Dynamics in Retinal Photoreceptors
    Authors: JY Chang, L Shi, ML Ko, GY Ko
    J. Biol. Rhythms, 2018;33(2):151-165.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  9. Expression of Pluripotency Markers in Non-pluripotent Human Neural Stem and Progenitor Cells
    Authors: PH Vincent, E Benedikz, P Uhlen, O Hovatta, E Sundström
    Stem Cells Dev., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration
    Authors: Q Wang, Y Liu, C Zhang, F Guo, C Feng, X Li, H Shi, Z Su
    Protein Expr. Purif, 2017;133(0):152-159.
    Species: Human
    Sample Types:
  11. Human bone marrow harbors cells with neural crest-associated characteristics like human adipose and dermis tissues
    Authors: C Coste, V Neirinckx, A Sharma, G Agirman, B Rogister, J Foguenne, F Lallemend, A Gothot, S Wislet
    PLoS ONE, 2017;12(7):e0177962.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Generation of neuropeptidergic hypothalamic neurons from human pluripotent stem cells.
    Authors: Merkle F, Maroof A, Wataya T, Sasai Y, Studer L, Eggan K, Schier A
    Development, 2015;142(4):633-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Human iPSC-derived motoneurons harbouring TARDBP or C9ORF72 ALS mutations are dysfunctional despite maintaining viability.
    Authors: Devlin, Anna-Cla, Burr, Karen, Borooah, Shyamang, Foster, Joshua D, Cleary, Elaine M, Geti, Imbisaat, Vallier, Ludovic, Shaw, Christop, Chandran, Siddhart, Miles, Gareth B
    Nat Commun, 2015;6(0):5999.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Autophagy induction enhances TDP43 turnover and survival in neuronal ALS models.
    Authors: Barmada, Sami J, Serio, Andrea, Arjun, Arpana, Bilican, Bilada, Daub, Aaron, Ando, D Michae, Tsvetkov, Andrey, Pleiss, Michael, Li, Xingli, Peisach, Daniel, Shaw, Christop, Chandran, Siddhart, Finkbeiner, Steven
    Nat Chem Biol, 2014;10(8):677-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Canine epidermal neural crest stem cells: characterization and potential as therapy candidate for a large animal model of spinal cord injury.
    Authors: Gericota B, Anderson J, Mitchell G, Borjesson D, Sturges B, Nolta J, Sieber-Blum M
    Stem Cells Transl Med, 2014;3(3):334-45.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Chicken embryos as a potential new model for early onset type I diabetes.
    Authors: Shi L, Ko M, Huang C, Park S, Hong M, Wu C, Ko G
    J Diabetes Res, 2014;2014(0):354094.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Involvement of ER stress in dysmyelination of Pelizaeus-Merzbacher Disease with PLP1 missense mutations shown by iPSC-derived oligodendrocytes.
    Authors: Numasawa-Kuroiwa Y, Okada Y, Shibata S, Kishi N, Akamatsu W, Shoji M, Nakanishi A, Oyama M, Osaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H
    Stem Cell Reports, 2014;2(5):648-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. A small molecule screen in stem-cell-derived motor neurons identifies a kinase inhibitor as a candidate therapeutic for ALS.
    Authors: Yang Y, Gupta S, Kim K, Powers B, Cerqueira A, Wainger B, Ngo H, Rosowski K, Schein P, Ackeifi C, Arvanites A, Davidow L, Woolf C, Rubin L
    Cell Stem Cell, 2013;12(6):713-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
    Authors: Zelinka C, Scott M, Volkov L, Fischer A
    PLoS ONE, 2012;7(9):e44477.
    Species: Chicken
    Sample Types: In Vivo
    Applications: In Vivo
  20. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2012;21(3):404-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Phosphatidylinositol 3 kinase-Akt signaling serves as a circadian output in the retina.
    Authors: Ko ML, Jian K, Shi L, Ko GY
    J. Neurochem., 2009;108(6):1607-20.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  22. Ciliary neurotrophic factor (CNTF) for human retinal degeneration: phase I trial of CNTF delivered by encapsulated cell intraocular implants.
    Authors: Sieving PA, Caruso RC, Tao W, Coleman HR, Thompson DJ, Fullmer KR, Bush RA
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(10):3896-901.
    Species: Human
    Sample Types: Serum
    Applications: ELISA (Capture)
  23. The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
    Authors: He W, Gong K, Smith DK, Ip NY
    FEBS Lett., 2005;579(20):4317-23.
    Species: Human
    Sample Types: Peptide
    Applications: Binding Assay
  24. Transitin, a nestin-related intermediate filament, is expressed by neural progenitors and can be induced in Muller glia in the chicken retina.
    Authors: Fischer AJ, Omar G
    J. Comp. Neurol., 2005;484(1):1-14.
    Species: Chicken
    Sample Types: In Vivo
    Applications: In Vivo
  25. PACAP promotes sensory neuron differentiation: blockade by neurotrophic factors.
    Authors: Nielsen KM, Chaverra M, Hapner SJ, Nelson BR, Todd V, Zigmond RE, Lefcort F
    Mol. Cell. Neurosci., 2004;25(4):629-41.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Membrane distal cytokine binding domain of LIFR interacts with soluble CNTFR in vitro.
    Authors: He W, Gong K, Zhu G, Smith DK
    FEBS Lett., 2002;514(2):214-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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