Recombinant Human CUL5/RBX2 Complex Protein, CF

R&D Systems | Catalog # E3-450

R&D Systems
Discontinued Product
E3-450 has been discontinued. View all CUL5/RBX2 Complex products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

Q93034, Q9UBF6

Applications

Bioactivity
View all CUL5/RBX2 Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL5/RBX2 Complex protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

94 kDa (CUL5), 13 kDa (RBX2)

Activity

Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.

Formulation, Preparation, and Storage

E3-450
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: CUL5/RBX2 Complex

Cullin-5 (CUL5) is a core component of multiple ECS (Elongin-CUL5-SOCS-box protein) E3 ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cytokine receptor signal transduction.  In the ECS complex, CUL5 serves as a scaffold that organizes the Elongin B/Elongin C/SOCS (Suppressor of Cytokine Signaling) recognition subunits with the RBX subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the ECS complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.  This complex consists of an N-terminal 10-His tagged CUL5 (UniProt Q93034) and untagged RBX2 (UniProt Q9UBF6).

References

  1. Baek K., et al. (2020) Nature  578: 461
  2. Duda D.M., et al. (2012) Mol. Cell  47: 371
  3. Kamura T., et al. (2004) Genes Dev.  18: 3055
  4. Kamura T., et al. (2001) J. Biol. Chem.  276: 29748

Long Name

Cullin 5/Ring Box Protein 2

UniProt

Q93034, Q9UBF6

Additional CUL5/RBX2 Complex Products

Product Documents for Recombinant Human CUL5/RBX2 Complex Protein, CF

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Product Specific Notices for Recombinant Human CUL5/RBX2 Complex Protein, CF

For research use only

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