Recombinant Human Endoglycan/PODXL2 Protein, CF
Recombinant Human Endoglycan/PODXL2 Protein, CF Summary
Gly33-Thr500, with a C-terminal 10-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Endoglycan, also called Podocalyxin-like 2 protein (PODXL2), is an extensively O-glycosylated type I transmembrane protein in the CD34 family of sialomucins (1, 2). Mature human Endoglycan consists of a 468 amino acid (aa) extracellular domain that contains an N-terminal acidic domain characterized by several polyglutamate tracts, a mucin domain, and a membrane-proximal globular domain. Within the extracellular domain, human Endoglycan shares 74% amino acid identity with both mouse and rat Endoglycan. Alternative splicing may generate a short isoform that lacks aa 336-411. Following the extracellular domain is a single transmembrane spanning helix and a charged cytoplasmic tail that contains a C-terminal PDZ-domain binding motif (1, 3). The cytoplasmic domain of Endoglycan has been shown to bind to Na+/H+ exchanger regulatory factor-1 (NHERF-1), which acts as a scaffolding protein that links membrane proteins to the actin cytoskeleton (4). Human Endoglycan exists as a disulfide-linked homodimer that is expressed in vascular endothelium, smooth muscle, hematopoietic precursor populations and leukocyte subpopulations (1, 5). Endoglycan functions through its interactions with selectins and is involved in leukocyte-endothelial cell adhesion although depending on cellular context and post-translational modifications, Endoglycan may also demonstrate anti-adhesive properties (3, 5).
- Sassetti, C. et al. (2000) J. Biol. Chem. 275:9001.
- Fieger, C.B. et al. (2003) J. Biol. Chem. 278:27390.
- Nielsen, J.S. & K.M. McNagny (2008) J. Cell Sci. 121:3683.
- Tan, P.C. et al. (2006) Stem Cells 24:1150.
- Kerr, S.C. et al. (2008) J. Immunol. 181:1480.
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