Recombinant Human Endoglycan/PODXL2 Protein, CF

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Recombinant Human Endoglycan/PODXL2 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED50 for this effect is 0.1-0.6 μg/mL.
Mouse myeloma cell line, NS0-derived human Endoglycan/PODXL2 protein
Gly33-Thr500, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
52 kDa
85-110 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Endoglycan/PODXL2

Human Endoglycan, also called Podocalyxin-like 2 protein (PODXL2), is an extensively O-glycosylated type I transmembrane protein in the CD34 family of sialomucins (1, 2). Mature human Endoglycan consists of a 468 amino acid (aa) extracellular domain that contains an N-terminal acidic domain characterized by several polyglutamate tracts, a mucin domain, and a membrane-proximal globular domain. Within the extracellular domain, human Endoglycan shares 74% amino acid identity with both mouse and rat Endoglycan. Alternative splicing may generate a short isoform that lacks aa 336-411. Following the extracellular domain is a single transmembrane spanning helix and a charged cytoplasmic tail that contains a C-terminal PDZ-domain binding motif (1, 3). The cytoplasmic domain of Endoglycan has been shown to bind to Na+/H+ exchanger regulatory factor-1 (NHERF-1), which acts as a scaffolding protein that links membrane proteins to the actin cytoskeleton (4). Human Endoglycan exists as a disulfide-linked homodimer that is expressed in vascular endothelium, smooth muscle, hematopoietic precursor populations and leukocyte subpopulations (1, 5). Endoglycan functions through its interactions with selectins and is involved in leukocyte-endothelial cell adhesion although depending on cellular context and post-translational modifications, Endoglycan may also demonstrate anti-adhesive properties (3, 5).


  1. Sassetti, C. et al. (2000) J. Biol. Chem. 275:9001.
  2. Fieger, C.B. et al. (2003) J. Biol. Chem. 278:27390.
  3. Nielsen, J.S. & K.M. McNagny (2008) J. Cell Sci. 121:3683.
  4. Tan, P.C. et al. (2006) Stem Cells 24:1150.
  5. Kerr, S.C. et al. (2008) J. Immunol. 181:1480.
Entrez Gene IDs
50512 (Human); 319655 (Mouse)
Alternate Names
Endoglycan; PODLX2; podocalyxin-like 2; podocalyxin-like protein 2; PODXL2


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