Recombinant Human FCRL3/FcRH3 Protein, CF

R&D Systems | Catalog # 3126-FC

R&D Systems
Discontinued Product
3126-FC has been discontinued. An alternative/replacement product is available: 3126-FCB. View all FCRL3/FcRH3 products.

Key Product Details

Source

NS0

Accession Number

NP_443171

Applications

Binding Activity
View all FCRL3/FcRH3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human FCRL3/FcRH3 protein
Arg14-Arg569, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg14

Predicted Molecular Mass

62.2 kDa

SDS-PAGE

80-90 kDa, reducing conditions

Activity

Measured by its ability to bind human IgG with an estimated KD <60 nM.

Reviewed Applications

Read 2 reviews rated 5 using 3126-FC in the following applications:

Formulation, Preparation, and Storage

3126-FC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FCRL3/FcRH3

FCRL3 (Fc Receptor-Like 3), also known as FcRH3, IRTA3, and SPAP2, is a 110 kDa molecule with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1). Mature human FCRL3 consists of a 556 amino acid (aa) extracellular domain (ECD) with six Ig-like domains, a 21 aa transmembrane segment, and a 140 aa cytoplasmic domain with four immunotyrosine inhibitory motifs (ITIMs) (2 - 4). Within the ECD, human and mouse FCRL3 share 35% aa sequence identity. Alternate splicing generates several additional isoforms with deletions or substitutions in both the extracellular and intracellular regions. These include potentially secreted forms that are truncated following the second Ig-like domain (4). FCRL3 is expressed in secondary lymphoid organs on the surface of mature naïve and memory B cells, NK cells, and B cell lines derived from chronic lymphocytic leukemias (2, 3, 5). It is upregulated on B cells following LPS or anti-CD40 stimulation (6). A polymorphism in the FCRL3 promoter induces enhanced transcription and is associated with the development of autoimmune disorders in a Japanese population (6, 7). Tyrosine phosphorylation within the ITIMs of FCRL3 enables its association with SHP-1 (4).

References

  1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
  2. Miller, I. et al. (2002) Blood, 99:2662.
  3. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
  4. Xu, M.-J. et al. (2002) Biochem. Biophys. Res. Commun. 293:1037.
  5. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  6. Kochi, Y. et al. (2005) Nat. Genet. 37:478.
  7. Chistiakov, D.A. and A.P. Chistiakov (2007) Hum. Immunol. 68:375.

Long Name

Fc Receptor-like 3

Alternate Names

CD307c, FcRH3, IFGP3, IRTA3, SPAP2

Entrez Gene IDs

115352 (Human); 329693 (Mouse); 680697 (Rat)

Gene Symbol

FCRL3

UniProt

NP_443171

Additional FCRL3/FcRH3 Products

Product Documents for Recombinant Human FCRL3/FcRH3 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human FCRL3/FcRH3 Protein, CF

For research use only

Citations for Recombinant Human FCRL3/FcRH3 Protein, CF

Customer Reviews for Recombinant Human FCRL3/FcRH3 Protein, CF (2)

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  • Recombinant Human FCRL3/FcRH3 Protein, CF
    Name: Gerre Lafond
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 02/19/2021
  • Recombinant Human FCRL3/FcRH3 Protein, CF
    Name: Alisha Freeman
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 10/09/2020

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