Recombinant Human Glycoprotein V/CD42d Protein, CF Summary
When 5 x 106 cells per well are added to rhGPV coated plates (5 μg/mL, 100 μL/well), approximately 50%-70% will adhere after 1 hour at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Gln17-Gly523, with a C-termninal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Glycoprotein V/CD42d
GPV (platelet glycoprotein V; designated CD42d) is an 83 kDa type I transmembrane (TM) glycoprotein of the leucine-rich repeat (LRR) family (1, 2). It is expressed exclusively within the platelet / megakaryocyte lineage, where it noncovalently interacts with other platelet TM LRR proteins, GPIb alpha / beta and GPIX, at a ratio of one GPV to two of each other subunit (2). The GPI-V-IX complex tethers platelets to von Willebrand factor on the surface of injured endothelial cells. Absence of the complex results in Bernard-Soulier syndrome, a rare bleeding disorder (1 - 3). The human GPV cDNA encodes a 560 amino acid (aa) protein with a 16 aa signal sequence, a 507 aa extracellular domain (ECD) containing 15 LRR, a 21 aa TM sequence, and a short (16 aa) cytoplasmic tail that binds calmodulin in resting, but not activated platelets. The human GPV ECD shares 70%, 71% and 81% aa identity with mouse, rat and equine GPV, respectively. GPV can form soluble fragments of 80 kDa by ADAM10 or ADAM17 cleavage after P507, or 69 kDa by thrombin cleavage after R476 (1, 4, 5). High circulating soluble GPV may be an indicator of platelet activation, but may also be caused by high doses of aspirin (6 - 8). The function of GPV is not entirely clear. Deletion of GPV in mice does not produce any obvious change to surface expression or function of GPIb and GPIX, but surface expression of GPV requires GPIb (9, 10). Deletion studies also indicate that GPV may play a minor role in collagen adhesion, and may modify platelet aggregation in response to thrombin (3, 11 - 15).
- Lanza, F. et al. (1993) J. Biol. Chem. 268:20801.
- Hickey, M.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8327.
- Ozaki, Y. et al. (2005) J. Thromb. Haemost. 3:1745.
- Rabie, T. et al. (2005) J. Biol. Chem. 280:14462.
- Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530.
- Wolff, V. et al. (2005) Stroke 36:e17.
- Javela, K. et al. (2005) Transfusion 45:1504.
- Aktas, B. et al. (2005) J. Biol. Chem. 280:39716.
- Kahn, M.L. (1999) Blood 94:4112.
- Strassel, C. et al. (2004) Eur. J. Biochem. 271:3671.
- Nonne, C. et al. (2008) J. Thromb. Haemost. 6:210.
- Moog, S. et al. (2001) Blood 98:1038.
- Ramakrishnan, V. et al. (1999) Proc. Natl. Acad. Sci. USA 96:13336.
- Ni, H. et al. (2001) Blood 98:368.
- Andrews, R.K. et al. (2001) Blood 98:681.
Citation for Recombinant Human Glycoprotein V/CD42d Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Glycoprotein V is a relevant immune target in patients with immune thrombocytopenia
Authors: R Vollenberg, R Jouni, PAA Norris, M Burg-Roder, N Cooper, MJ Rummel, G Bein, I Marini, B Bayat, R Burack, AH Lazarus, T Bakchoul, UJ Sachs
Sample Types: Serum
Applications: Surface Plasmon Resonance (SPR
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