Recombinant Human GST-MuRF1/TRIM63 Protein, CF

Recombinant Human GST-MuRF1/TRIM63 Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Keap1

Muscle-specific RING-finger Protein 1 (MuRF1, also known as TRIM63) is an E3 Ubiquitin ligase and member of a family of tripartite motif (TRIM) proteins containing more than 60 members in metazoans.  Human TRIM63 is 353 amino acids in length with a predicted molecular weight of 40 kDa and is 88% identical to the mouse and rat orthologs.  TRIM63 contains two zinc finger domains (one RING type and one B-Box type), a coiled-coil region, and a C-terminal COS domain.  It is expressed primarily in striated muscle, and evidence suggests it may target sarcomeric proteins for ubiquitination and subsequent proteasome-dependent degradation.  Target proteins for TRIM63 may include Actin, Myosin-binding Protein C, and Myosin Light Chains 1 and 2.  TRIM63 has been implicated in muscular atrophy associated with chronic disease and inactivity.  This recombinant protein contains an N-terminal GST tag.

Related Research Areas

• Stem Cell Transcription Factor Regulators
• Transcriptional Co-Repressors