Recombinant Human GST-MuRF1/TRIM63 Protein, CF

R&D Systems | Catalog # E3-308

R&D Systems
Discontinued Product
E3-308 has been discontinued. View all Keap1 products.

Key Product Details

Source

E. coli

Accession Number

Q969Q1

Applications

Bioactivity
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Product Specifications

Source

E. coli-derived human MuRF1/TRIM63 protein

Purity

>70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

67 kDa

Activity

Recombinant Human GST-MuRF1/TRIM63 is an E3 Ubiquitin Ligase that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial TRIM63 concentration of 50-500 nM.

Formulation, Preparation, and Storage

E3-308
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol,      1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Keap1

Muscle-specific RING-finger Protein 1 (MuRF1, also known as TRIM63) is an E3 Ubiquitin ligase and member of a family of tripartite motif (TRIM) proteins containing more than 60 members in metazoans.  Human TRIM63 is 353 amino acids in length with a predicted molecular weight of 40 kDa and is 88% identical to the mouse and rat orthologs.  TRIM63 contains two zinc finger domains (one RING type and one B-Box type), a coiled-coil region, and a C-terminal COS domain.  It is expressed primarily in striated muscle, and evidence suggests it may target sarcomeric proteins for ubiquitination and subsequent proteasome-dependent degradation.  Target proteins for TRIM63 may include Actin, Myosin-binding Protein C, and Myosin Light Chains 1 and 2.  TRIM63 has been implicated in muscular atrophy associated with chronic disease and inactivity.  This recombinant protein contains an N-terminal GST tag.

References

  1. Ozata, K. et al. (2008) Nat. Rev. Immunol.  8: 849
  2. Nakada, S. et al. (2010) Nature  446: 941
  3. Sato, Y. et al. (2012) J. Biol. Chem.  287: 25860
  4. Wang, T. et al. (2009)  J. Mol. Biol.  386: 1011
  5. Wiener, R. et al. (2012) Nature  483: 618

Long Name

Kelch-like ECH-associated Protein 1

Alternate Names

INrf2

Entrez Gene IDs

9817 (Human)

Gene Symbol

KEAP1

UniProt

Q969Q1 (Human)

Additional Keap1 Products

Product Documents for Recombinant Human GST-MuRF1/TRIM63 Protein, CF

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Product Specific Notices for Recombinant Human GST-MuRF1/TRIM63 Protein, CF

For research use only

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