Recombinant Human His6-Ataxin UIM Domains Protein, CF

R&D Systems | Catalog # UBE-100

R&D Systems
Discontinued Product
UBE-100 has been discontinued. View all Ataxin UIM Domains products.

Key Product Details

Source

E. coli

Accession Number

P54252-1

Applications

Enzyme Activity
View all Ataxin UIM Domains Proteins and Enzymes »

Product Specifications

Source

E. coli-derived

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Activity

His6-Ataxin UIM can be used for the purification and identification of K48- or K63-linked Ubiquitin chains. Reaction conditions will need to be optimized for each specific application. We recommend using 50-100 μg of His6-Ataxin UIM to detect 10-20 μg of purified Ubiquitin chains.

Formulation, Preparation, and Storage

UBE-100
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Ataxin UIM Domains

The Ubiquitin-interacting Motif (UIM) is an alpha-helical Ubiquitin-binding domain found in many proteins that recognizes and traffics ubiquitinated cargo (1,2). Ataxin-3, a deubiquitinating enzyme that contains three consecutive UIM motifs (amino acids (aa) 224-243, 244-263, 331-348), functions as a mixed lineage, chain editing enzyme that recognizes and binds K48-linked and K63-linked poly-Ubiquitin chains (3-5). The UIM domain of Ataxin-3 (aa 224-348) preferentially interacts with four or more Ubiquitin units of K48-linked or K63-linked poly-Ubiquitin chains or ubiquitinated substrates that contain these linkages (4,6,7).

 

This protein can be used for the isolation and identification of K48-linked (preferentially) or K63-linked poly-Ubiquitin chains or ubiquitinated substrates that contain these linkages. This protein is His-tagged which allows for metal chelate affinity purification and also allows for convenient immuno-detection of conjugates using His6-specific antibodies. 

References

  1. Sgourakis, N.G. et al. (2010) J. Mol. Biol. 396:1128.
  2. Hurley, J.H. et al. (2006) Biochem. J. 399:361.
  3. Albrecht, M. et al. (2004) Eur. J. Biochem. 271:3155.
  4. Burnett, B. et al. (2003) Hum. Mol. Genet. 12:3195.
  5. Mao, Y. et al. (2005) Proc. Natl. Acad. Sci. USA 102:12700.
  6. Berke, S.J. et al. (2005) J. Biol. Chem. 280:32026.
  7. Winborn, B.J. et al. (2008) J. Biol. Chem. 283:26436.
 

Entrez Gene IDs

4287 (Human); 110616 (Mouse); 60331 (Rat)

Gene Symbol

ATXN3

UniProt

P54252-1

Product Documents for Recombinant Human His6-Ataxin UIM Domains Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6-Ataxin UIM Domains Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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