Recombinant Human His6-Ataxin UIM Domains Protein, CF

Recombinant Human His6-Ataxin UIM Domains Protein, CF Summary

Specifications

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Background: Ataxin UIM Domains

The Ubiquitin-interacting Motif (UIM) is an alpha-helical Ubiquitin-binding domain found in many proteins that recognizes and traffics ubiquitinated cargo (1,2). Ataxin-3, a deubiquitinating enzyme that contains three consecutive UIM motifs (amino acids (aa) 224-243, 244-263, 331-348), functions as a mixed lineage, chain editing enzyme that recognizes and binds K48-linked and K63-linked poly-Ubiquitin chains (3-5). The UIM domain of Ataxin-3 (aa 224-348) preferentially interacts with four or more Ubiquitin units of K48-linked or K63-linked poly-Ubiquitin chains or ubiquitinated substrates that contain these linkages (4,6,7).

This protein can be used for the isolation and identification of K48-linked (preferentially) or K63-linked poly-Ubiquitin chains or ubiquitinated substrates that contain these linkages. This protein is His-tagged which allows for metal chelate affinity purification and also allows for convenient immuno-detection of conjugates using His6-specific antibodies. 

Related Research Areas

• ALS Related Molecules
• Deubiquitinating Enzymes (DUBs)