Recombinant Human His6-Fluorescein-Tandem UBA (TUBE1), CF

R&D Systems | Catalog # UBE-212

R&D Systems
Discontinued Product
UBE-212 has been discontinued.

Key Product Details

Species

Human

Applications

Bioactivity

Accession Number

P54725

Conjugate

Fluorescein

Formulation, Preparation, and Storage

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background:

Human HR23A/Rad23A has two Ubiquitin-associated (UBA) motifs that can each bind Ubiquitin via a hydrophobic surface formed by residues located within the alpha 1 and alpha 3 helices of each UBA domain. Tandem-repeated Ubiquitin binding entities (TUBEs), which consist of multiple tandem Ubiquitin-binding UBA motifs, have been developed for the isolation and identification of ubiquitinated proteins. TUBEs show increased affinity for poly-Ubiquitin moieties compared to single UBA motifs. Additionally, TUBEs protect polyubiquitinated proteins from deubiquitylating enzymes, allowing for the detection of polyubiquitinated proteins at relatively low levels of abundance.

References

  1. Chen, L. et al. (2001) EMBO Rep. 2:933.
  2. Wang, Q. et al. (2003) Biochemistry 42:13529.
  3. Hjerpe, R. et al. (2009) EMBO Rep. 10:1250.
  4. Hurley, J.H. et al. (2006) Biochem. J. 399:361.
  5. Lopitz-Otsoa, F. et al. (2010) Biochem. Soc. Trans. 38:40.

UniProt

P54725

Product Documents for Recombinant Human His6-Fluorescein-Tandem UBA (TUBE1), CF

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Product Specific Notices for Recombinant Human His6-Fluorescein-Tandem UBA (TUBE1), CF

For research use only

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