Recombinant Human His6-HSP40/DNAJB1 Protein, CF

Catalog # Availability Size / Price Qty
AP-110-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
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Recombinant Human His6-HSP40/DNAJB1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Source
E. coli-derived human HSP40/DNAJB1 protein
Contains a C-terminal 6-His tag
Accession #
Predicted Molecular Mass
39 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AP-110

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: HSP40/DNAJB1

Heat shock protein 40 (HSP40, also known as DNAJB1) is the human homologue of the bacterial DnaJ heat shock protein. Heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms, and they are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. HSP40 is a stress inducible chaperone that co­localizes with HSP70 and can bind unfolded proteins and prevent protein denaturation and aggregation. The conserved amino terminal J domain can interact with HSP70 and stimulate its ATPase activity. HSP40 and HSP70 are required to target some misfolded proteins to the Ubiquitin E3 ligase CHIP (Stub1) for subsequent ubiquitination.  This recombinant human protein contains a C-terminal His6 tag.

References
  1. Chien V., et. al. (2010) Biochem J. 432: 113-121
  2. Hinault M.P., et. al. (2010) J Biol. Chem. 285: 38173-38182
  3. Minami Y., et. al. (1996) J Biol. Chem. 271: 19617-19624
  4. Qiu X.B., et. al. (2006) Cell Mol. Life. Sci. 63: 2560-2570
Long Name
Heat Shock 40 kDa Protein
Entrez Gene IDs
3337 (Human); 81489 (Mouse); 361384 (Rat)
Alternate Names
DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ (Hsp40) homolog, subfmaily B, member 1; dnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB1; Hdj1; hDj-1; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; Heat shock protein 40; HSP40; HSPF1; HSPF1Hdj1; Human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1

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Recombinant Human His6-HSP40/DNAJB1 Protein, CF
By Anonymous on 08/17/2018
Application: In vitro bioactivity in cell culture