Recombinant Human His6-HSP40/DNAJB1 Protein, CF

Please inquire regarding future lot manufacture; Will be discontinued when existing inventory is gone.

Catalog # Availability Size / Price Qty

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Reviews (1)

Recombinant Human His6-HSP40/DNAJB1 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
E. coli-derived human HSP40/DNAJB1 protein
Met1 - Thr340 with a C-terminal 6-His tag
Accession #
Predicted Molecular Mass
39 kDa

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a solution in HEPES, NaCl and TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: HSP40/DNAJB1

Heat shock protein 40 (HSP40, also known as DNAJB1) is the human homologue of the bacterial DnaJ heat shock protein. Heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms, and they are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. HSP40 is a stress inducible chaperone that co­localizes with HSP70 and can bind unfolded proteins and prevent protein denaturation and aggregation. The conserved amino terminal J domain can interact with HSP70 and stimulate its ATPase activity. HSP40 and HSP70 are required to target some misfolded proteins to the Ubiquitin E3 ligase CHIP (Stub1) for subsequent ubiquitination.  

  1. Chien, V., et. al. (2010) Biochem. J. 432:113.
  2. Hinault, M.P., et. al. (2010) J. Biol. Chem. 285:38173.
  3. Minami, Y., et. al. (1996) J. Biol. Chem. 271:19617.
  4. Qiu, X.B., et. al. (2006) Cell Mol. Life Sci. 63: 2560.
Long Name
Heat Shock 40 kDa Protein
Entrez Gene IDs
3337 (Human); 81489 (Mouse); 361384 (Rat)
Alternate Names
DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ (Hsp40) homolog, subfmaily B, member 1; dnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB1; Hdj1; hDj-1; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; Heat shock protein 40; HSP40; HSPF1; HSPF1Hdj1; Human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human His6-HSP40/DNAJB1 Protein, CF

Average Rating: 5 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Recombinant Human His6-HSP40/DNAJB1 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Recombinant Human His6-HSP40/DNAJB1 Protein, CF
By Neda Ghousifam on 08/17/2018
Application: In vitro bioactivity in cell culture