Recombinant Human His6-Josephin-1 Protein, CF

R&D Systems | Catalog # E-618

R&D Systems
Discontinued Product
E-618 has been discontinued. View all Josephin-1/JOSD1 products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

Q15040

Applications

Bioactivity
View all Josephin-1/JOSD1 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Josephin-1/JOSD1 protein
Contains a c-terminal 6His tag.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

24 kDa

Activity

Recombinant Human Josephin-1 is a Ubiquitin-specific deconjugating enzyme.  Reaction Conditions will need to be optimized for each specific application.  We recommend an initial Josephin-1 concentration of 10-100 nM when using Ubiquitin-rhodamine (U-555) substrate and 100-500 nM when using Ubiquitin-AMC (U-550) substrate.  As supplied, this enzyme will not efficiently cleave purified recombinant polyubiquitin chains.

Reviewed Applications

Read 1 review rated 4 using E-618 in the following applications:

Formulation, Preparation, and Storage

E-618
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.0, 300 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Josephin-1/JOSD1

Josephin-1 is a member of the Machado-Joseph Disease (MJD) family of cysteine proteases, whose other members include Ataxin-3, Ataxin-3-like, and Josephin-2.  202 amino acids long with a predicted molecular weight of 23 kDa, Josephin-1 shares 97% identity with its mouse and rat orthologues.  When ubiquitinated, Josephin-1 is capable of cleaving K48- and K63-linked polyubiquitin chains in vitro.  Biologically, this enzyme may act to increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis.

References

  1. Seki T. et al.  (2013) J. Biol. Chem.  288: 17145
  2. Weeks S.D. et al.  (2011) J. Biol. Chem.  286: 4555

Long Name

Josephin Domain Containing 1

Alternate Names

EC 3.4.19.12, JOSD1, JSPH1

Entrez Gene IDs

9929 (Human); 74158 (Mouse); 315134 (Rat)

Gene Symbol

JOSD1

UniProt

Q15040

Additional Josephin-1/JOSD1 Products

Product Documents for Recombinant Human His6-Josephin-1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6-Josephin-1 Protein, CF

For research use only

Citations for Recombinant Human His6-Josephin-1 Protein, CF

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    Application: Enzymatic activity in vitro
    Verified Customer | Posted 01/21/2021

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