Recombinant Human His6-Livin beta Protein, CF

R&D Systems | Catalog # E3-300

R&D Systems
Discontinued Product
E3-300 has been discontinued. View all Livin products.

Key Product Details

Source

E. coli

Accession Number

Q96CA5-2

Applications

Bioactivity
View all Livin Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Livin protein
Contains a C-terminal 6-His tag.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

32 kDa

Activity

Recombinant Human Livin beta is a Ubiquitin Ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.

Formulation, Preparation, and Storage

E3-300
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Livin

Livin alpha and Livin beta are two splice variants generated from the Baculoviral IAP repeat-containing protein 7 (BIRC7) gene.  Livin in a member of the Inhibitor of Apoptosis Protein (IAP) family that contains a single baculovirus IAP repeat (BIR) and a C-terminal RING domain.  Livin exerts an anti-apoptotic activity by inhibiting caspase's 3, 7, and 9, and by its E3 Ubiquitin ligase activity.  In vitro, Livin directly inhibits caspase 3 and proteolytic activation of pro-caspase 9, and ubiquitinates recombinant DIABLO.  Livin beta is a splice variant that's 18 amino acids shorter in the BIR-RING linking region than the Livin alpha splice variant.

References

  1. Ashab, Y. et al. (2001) FEBS  495: 56
  2. Kasof, G.M. & Gomes B.C. (2001) J. Biol. Chem.  276: 3238
  3. Lin, J-H. et al. (2000) Biochem. Biophys. Res. Comm.  279: 820
  4. Ma, L. et al. (2006) Cell Death Differ.  13: 2079
  5. Vucic, D. et al. (2000) Curr. Biol.  10: 1359

Alternate Names

BIRC7, KIAP, ML-IAP

Entrez Gene IDs

79444 (Human)

Gene Symbol

BIRC7

UniProt

Q96CA5-2

Additional Livin Products

Product Documents for Recombinant Human His6-Livin beta Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6-Livin beta Protein, CF

For research use only

Related Research Areas

Cysteine Proteases and Regulators

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail