Recombinant Human His6-Livin beta Protein, CF

Catalog # Availability Size / Price Qty
E3-300-050
Product Details
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Recombinant Human His6-Livin beta Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human Livin beta is a Ubiquitin Ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.
Source
E. coli-derived human Livin protein
Contains a C-terminal 6-His tag.
Accession #
Predicted Molecular Mass
32 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-300

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: Livin

Livin alpha and Livin beta are two splice variants generated from the Baculoviral IAP repeat-containing protein 7 (BIRC7) gene.  Livin in a member of the Inhibitor of Apoptosis Protein (IAP) family that contains a single baculovirus IAP repeat (BIR) and a C-terminal RING domain.  Livin exerts an anti-apoptotic activity by inhibiting caspase's 3, 7, and 9, and by its E3 Ubiquitin ligase activity.  In vitro, Livin directly inhibits caspase 3 and proteolytic activation of pro-caspase 9, and ubiquitinates recombinant DIABLO.  Livin beta is a splice variant that's 18 amino acids shorter in the BIR-RING linking region than the Livin alpha splice variant.

References
  1. Ashab, Y. et al. (2001) FEBS  495: 56
  2. Kasof, G.M. & Gomes B.C. (2001) J. Biol. Chem.  276: 3238
  3. Lin, J-H. et al. (2000) Biochem. Biophys. Res. Comm.  279: 820
  4. Ma, L. et al. (2006) Cell Death Differ.  13: 2079
  5. Vucic, D. et al. (2000) Curr. Biol.  10: 1359
Entrez Gene IDs
79444 (Human)
Alternate Names
baculoviral IAP repeat containing 7; baculoviral IAP repeat-containing 7; BIRC7; KIAP; KIAPRING finger protein 50; Kidney inhibitor of apoptosis protein; livin inhibitor-of-apoptosis; Livin; Melanoma inhibitor of apoptosis protein; ML-IAP; MLIAPlivin inhibitor of apoptosis; ML-IAPmliap; RNF50LIVINbaculoviral IAP repeat-containing protein 7

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