NEDD8-specific Protease 1 (NEDP1), also known as SUMO/Sentrin Specific Peptidase Family Member 8 (SENP8), DEN1, PRSC2, and FKSG8, is a cytoplasmic cysteine protease that belongs to the ULP/peptidase C48 family of deubiquitinating enzymes (1). It is widely expressed with the highest levels being observed in kidney and pancreas (2). NEDP1/SENP8 is 212 amino acids (aa) in length with a predicted molecular weight of 24.1 kDa (1). It possesses an acetylated initiating methionine plus a protease domain that spans aa 11-174. Human NEDP1/SENP8 shares 92% aa sequence identity with the mouse and rat orthologs. NEDP1/SENP8 is involved in NEDD8-associated ubiquitination (1,2,3-5). NEDD8 is a small Ubiquitin-like protein that covalently binds to target molecules, including Cullin 1 and p53 (6-8). It is a critical regulator of cell proliferation and development (3). NEDP1/SENP8 influences the NEDDylation of proteins by converting pro-NEDD8 into its mature form and deconjugating NEDD8 from its bound substrates (2,3-5,9).
Discontinued Product
E-800 has been discontinued.
View all NEDP1/SENP8 products.
Key Product Details
Source
E. coli
Accession Number
Applications
Enzyme Activity
Loading...
Product Specifications
Source
E. coli-derived human NEDP1/SENP8 protein
Contains a 6-His tag
Contains a 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
27 kDa
Activity
Recombinant Human His6-NEDP1/SENP8 is a NEDD8-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-NEDP1/SENP8 concentration of 50-500 nM. A 15 minute pre-incubation with 10 mM DTT is recommended to achieve maximum activity.
Reviewed Applications
Read 1 review rated 5 using E-800 in the following applications:
Formulation, Preparation, and Storage
E-800
| Formulation | X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 2 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: NEDP1/SENP8
References
- Gan-Erdene, T. et al. (2003) J. Biol. Chem. 278:28892.
- Mendoza, H.M. et al. (2003) J. Biol. Chem. 278:25637.
- Wu, K. et al. (2003) J. Biol. Chem. 278:28882.
- Shen, L. et al. (2005) EMBO J. 24:1341.
- Shin, Y.C. et al. (2011) PLoS One 6:e27742.
- Pan, Z.Q. et al. (2004) Oncogene 23:1985.
- Xirodimas, D.P. et al. (2004) Cell 118:83.
- Xirodimas, D.P. et al. (2008) EMBO Rep. 9:280.
- Ehrentraut, S.F. et al. (2012) J. Immunol. [Epub ahead of print].
Alternate Names
DEN1, Deneddylase-1, PRSC2, SENP8
Gene Symbol
SENP8
UniProt
Additional NEDP1/SENP8 Products
Product Documents for Recombinant Human His6-NEDP1/SENP8 Protein, CF
Product Specific Notices for Recombinant Human His6-NEDP1/SENP8 Protein, CF
For research use only
Related Research Areas
Customer Reviews for Recombinant Human His6-NEDP1/SENP8 Protein, CF (1)
5 out of 5
1 Customer Rating
Have you used Recombinant Human His6-NEDP1/SENP8 Protein, CF?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Showing
1
-
1 of
1 review
Showing All
Filter By:
-
Application: Binding assay/Protein-protein interactionVerified Customer | Posted 11/01/2017
There are no reviews that match your criteria.