Recombinant Human His6-UBE4B Protein, CF

R&D Systems | Catalog # E3-270

R&D Systems
Discontinued Product
E3-270 has been discontinued. View all UBE4B products.

Key Product Details

Source

Sf 21 (baculovirus)

Applications

Bioactivity
View all UBE4B Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human UBE4B protein
Contains an N-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

147 kDa

Activity

Typical enzyme concentration for in vitro conjugation reactions is 100-500 nM depending on experimental conditions and substrate.  Recombinant human UBE4B demonstrates strong in vitro autoubiquitination activity in the presence of UBE1, UBE2D3, Ubiquitin, and ATP.

Formulation, Preparation, and Storage

E3-270
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 20% (v/v) Glycerol, 1 mM TCEP, 10 μM ZnCl2
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: UBE4B

Ubiquitin conjugation factor E4 B (UBE4B, also known as Ubiquitin fusion degradation protein 2) is a recently identified E3 and E4 Ubiquitin ligase that physically interacts with p53 and Hdm2 to promote p53 polyubiquitination and degradation.  Recent work in mouse models suggests that p53 loss in accelerated medulloblastomas is driven by UBE4B.  Overexpression of UBE4B correlated with decreased expression of p53 in xenotransplant tumor models, and overexpression of UBE4B is often observed in human brain tumors.  UBE4B appears to be involved in multiple pathways that are associated with neuronal survival.

References

  1. Gillingwater T.H. et al. (2002) J. Physiol.  543: 739
  2. Wu H. et al. (2011) Nat. Med.  3: 347
  3. Wu H. & Leng R.P. (2011) Cell Cycle  10: 1912
  4. Zeinab R.A. et al. (2012) Int. J. Mol. Sci.  12: 16865

Long Name

Ubiquitination Factor E4B

Alternate Names

UBOX3, UFD2, UFD2a

Entrez Gene IDs

10277 (Human); 63958 (Mouse)

Gene Symbol

UBE4B

Additional UBE4B Products

Product Documents for Recombinant Human His6-UBE4B Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6-UBE4B Protein, CF

For research use only

Related Research Areas

E3 Ubiquitin Ligases

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