Recombinant Human His6-UBE4B Protein, CF

Catalog # Availability Size / Price Qty
E3-270-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human His6-UBE4B Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical enzyme concentration for in vitro conjugation reactions is 100-500 nM depending on experimental conditions and substrate.  Recombinant human UBE4B demonstrates strong in vitro autoubiquitination activity in the presence of UBE1, UBE2D3, Ubiquitin, and ATP.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human UBE4B protein
Contains an N-terminal 6-His tag
Predicted Molecular Mass
147 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-270

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 20% (v/v) Glycerol, 1 mM TCEP, 10 μM ZnCl2
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE4B

Ubiquitin conjugation factor E4 B (UBE4B, also known as Ubiquitin fusion degradation protein 2) is a recently identified E3 and E4 Ubiquitin ligase that physically interacts with p53 and Hdm2 to promote p53 polyubiquitination and degradation.  Recent work in mouse models suggests that p53 loss in accelerated medulloblastomas is driven by UBE4B.  Overexpression of UBE4B correlated with decreased expression of p53 in xenotransplant tumor models, and overexpression of UBE4B is often observed in human brain tumors.  UBE4B appears to be involved in multiple pathways that are associated with neuronal survival.

References
  1. Gillingwater T.H. et al. (2002) J. Physiol.  543: 739
  2. Wu H. et al. (2011) Nat. Med.  3: 347
  3. Wu H. & Leng R.P. (2011) Cell Cycle  10: 1912
  4. Zeinab R.A. et al. (2012) Int. J. Mol. Sci.  12: 16865
Long Name
Ubiquitination Factor E4B
Entrez Gene IDs
10277 (Human); 63958 (Mouse)
Alternate Names
E4; homologous to yeast UFD2; Homozygously deleted in neuroblastoma 1; homozygously deleted in neuroblastoma-1; KIAA0684HDNB1; UBE4B; ubiquitin conjugation factor E4 B; Ubiquitin fusion degradation protein 2; ubiquitination factor E4B (homologous to yeast UFD2); ubiquitination factor E4B (UFD2 homolog, yeast); ubiquitin-fusion degradation protein 2; UBOX3; UFD2; UFD2a; UFD2UFD2A

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