Recombinant Human His6-USP11 Protein, CF

R&D Systems | Catalog # E-602

R&D Systems
Discontinued Product
E-602 has been discontinued. View all USP11 products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

P51784

Applications

Bioactivity
View all USP11 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP11 protein
Contains an C-terminal 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

111 kDa

Activity

Reaction conditions will need to be optimized for each specific application.  We recommend an initial recombinant human USP11 concentration of 10-50 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550, U-555).

Formulation, Preparation, and Storage

E-602
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol,  1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP11

Ubiquitin carboxyl-terminal hydrolase 11 (USP11) is a specialized cysteine protease with a predicted molecular weight of 110 kDa. USP11 is a member of the peptidase C19 family and the human protein shares 77% amino acid sequence identity with its mouse ortholog. USP11 plays a role in DNA double-strand break (DSB) repair, and cells are hypersensitive to gamma radiation when USP11 has been knocked down. USP11 overexpression is often observed in colorectal cancer and melanoma and is correlated with poor survival. In these cases USP11 may be driving disease states by stabilizing cIAP2. Interestingly, recent genetic analysis suggests that USP11 was generated during the course of vertebrate evolution by a small-scale duplication of the USP4-encoding region. This recombinant protein contains a C-terminal 6-His tag.

References

  1. Lee, E.W. et al. (2015) Cell Death and Differ.  22: 1463
  2. Vlasschaert, C. et al. (2015) BMC Evol. Biol. 15: 230
  3. Yu, M. et al. (2016) J.Biol.Chem. 291: 959

Long Name

Ubiquitin Specific Protease 11

Alternate Names

Ubiquitin Thioesterase 11, UHX1

Entrez Gene IDs

8237 (Human); 236733 (Mouse); 408217 (Rat)

Gene Symbol

USP11

UniProt

P51784

Additional USP11 Products

Product Documents for Recombinant Human His6-USP11 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6-USP11 Protein, CF

For research use only

Citations for Recombinant Human His6-USP11 Protein, CF

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