Recombinant Human His6-USP13 Protein, CF

R&D Systems | Catalog # E-588

R&D Systems
Discontinued Product
E-588 has been discontinued. View all USP13 products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

Q92995

Applications

Bioactivity
View all USP13 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP13 protein
Contains an C-terminal 6-His tag

Purity

>98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

98 kDa

Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP13 concentration of 100-500 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)

Formulation, Preparation, and Storage

E-588
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP13

Ubiquitin carboxyl-terminal hydrolase 13 (USP13) is a specialized cysteine protease with a predicted molecular weight of 97 kDa. USP13 is a member of the peptidase C19 family and the human protein shares 96% amino acid sequence identity with its mouse ortholog. Recombinant USP13 has weak deubiquitinase activity against unanchored polyubiquitin chains, but will disassemble tetraubiquitin chains in extended reactions, particularly K63 and K48 linkages.  Suggested biological targets of USP13 include PTEN, BECN1, MITF, SKP2 and USP10, and roles for USP13 in melanoma and colorectal cancer have been reported. This recombinant protein contains a C-terminal 6-His tag.

References

  1. Xiang, S. et al. (2015) Oncol Rep. 33: 1342
  2. Zhang, J. et al. (2013) Nat Cell Biol. 15: 1486
  3. Zhang, Y-H. et al. (2011) PLoS One 6: e29362
  4. Zhao, X. et al. (2011) Nat Commun. 2: 414. doi: 10.1038/ncomms1421

Long Name

Ubiquitin Specific Protease 13

Alternate Names

Isopeptidase T-3, IsoT-3, Ubiquitin Thioesterase 13

Entrez Gene IDs

8975 (Human); 72607 (Mouse); 310306 (Rat)

Gene Symbol

USP13

UniProt

Q92995

Additional USP13 Products

Product Documents for Recombinant Human His6-USP13 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human His6-USP13 Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Recombinant Human His6-USP13 Protein, CF

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