Recombinant Human His6-USP16 Protein, CF

R&D Systems | Catalog # E-612

R&D Systems
Discontinued Product
E-612 has been discontinued. View all USP16 products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

Q9Y5T5

Applications

Bioactivity
View all USP16 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP16 protein

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

94 kDa

Activity

Recombinant Human USP16 is a Ubiquitin-specific deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  USP16 does not efficiently utilize Ubiquitin-AMC (U-550), Ubiquitin-Rhodamine 110 (U-555), nor unanchored poly-Ubiquitin chains as substrates in vitro.  USP16 cleaves Ubiquitin from recombinant, designer nucleosomes. 

Reviewed Applications

Read 1 review rated 5 using E-612 in the following applications:

Formulation, Preparation, and Storage

E-612
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP16

USP16 is a deubiquitinating enzyme (DUB) of the C19 peptidase family, USP16 subfamily.  Human USP16 has a predicted molecular weight of 94 kDa and is 83% identical to both mouse and rat orthologues.  USP16 plays significant roles in cell-cycle progression and Hox gene expression, and USP16 knockdown in HeLa cells results in defects in the mitotic phase of the cell cycle.  Studies reveal that histone H2A deubiquitination by USP16 is a prerequisite for phosphorylation of serine 10 of histone H3 and chromosome segregation during mitosis.  USP16 specifically deubiquitinates histone H2A, but not H2B, both in vitro and in vivo.  PLK1 serine/threonine kinase is another reported target of USP16.  Finally, a chromosomal inversion involving USP16 and RUNX1/AML1 is a causative event in the development of chronic myelomonocytic leukemia (CMML).  This recombinant protein contains an N-terminal 6-His tag.

References

  1. Cai S.Y., Babbitt R.W., Marchesi V.T. (1999) Proc. Natl. Acad. Sci.  96: 2828
  2. Joo H.Y., et al. (2007) Nature  499: 1068
  3. Gelsi-Boyer V., et al. (2008)  BMC Cancer  8: 299
  4. Zhuo X., et al. (2015) J. Cell Biol.  210: 727

Long Name

Ubiquitin Specific Protease 16

Alternate Names

UBP-M

Entrez Gene IDs

10600 (Human); 74112 (Mouse); 288306 (Rat)

Gene Symbol

USP16

UniProt

Q9Y5T5

Additional USP16 Products

Product Documents for Recombinant Human His6-USP16 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human His6-USP16 Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Recombinant Human His6-USP16 Protein, CF

Customer Reviews for Recombinant Human His6-USP16 Protein, CF (1)

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  • Recombinant Human His6-USP16 Protein, CF
    Name: valentina rossio
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 01/21/2021

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