Recombinant Human His6-USP16 Protein, CF
Recombinant Human His6-USP16 Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
USP16 is a deubiquitinating enzyme (DUB) of the C19 peptidase family, USP16 subfamily. Human USP16 has a predicted molecular weight of 94 kDa and is 83% identical to both mouse and rat orthologues. USP16 plays significant roles in cell-cycle progression and Hox gene expression, and USP16 knockdown in HeLa cells results in defects in the mitotic phase of the cell cycle. Studies reveal that histone H2A deubiquitination by USP16 is a prerequisite for phosphorylation of serine 10 of histone H3 and chromosome segregation during mitosis. USP16 specifically deubiquitinates histone H2A, but not H2B, both in vitro and in vivo. PLK1 serine/threonine kinase is another reported target of USP16. Finally, a chromosomal inversion involving USP16 and RUNX1/AML1 is a causative event in the development of chronic myelomonocytic leukemia (CMML). This recombinant protein contains an N-terminal 6-His tag.
- Cai S.Y., Babbitt R.W., Marchesi V.T. (1999) Proc. Natl. Acad. Sci. 96: 2828
- Joo H.Y., et al. (2007) Nature 499: 1068
- Gelsi-Boyer V., et al. (2008) BMC Cancer 8: 299
- Zhuo X., et al. (2015) J. Cell Biol. 210: 727
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