Recombinant Human His6-USP19 Protein, CF

Catalog # Availability Size / Price Qty
E-576-050
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Recombinant Human His6-USP19 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity

Recombinant Human His6-USP19 is a ubiquitin­-specific deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application. We recommend an initial USP19 concentration of 10-100 nM.

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP19 protein
Met1 - Arg1290, Contains a C-terminal 6-His tag
Accession #
Predicted Molecular Mass
143 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-576

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: USP19

Ubiquitin specific processing protease 19 (USP19) is a 146 kDa member of the C19 peptidase family that contains two alpha crystallin domains and one MYND-type zinc finger. USP19 plays a role in the rescue of ER-associated protein degradation (ERAD) substrates such as cystic fibrosis transmembrane conductance regulator (CFTR) delta F508 and T-cell receptor-alpha (TCR alpha ) from proteasomal degradation. USP19 also interacts with the ubiquitin ligases cellular IAP 1 (cIAP1) and cIAP2. Knockdown of USP19 decreases levels of both cIAPs, whereas overexpression of USP19 results in a marked increase in cIAP levels. Although it effectively removes ubiquitin from cIAPs in vitro, USP19 may stabilize cIAP proteins in vivo mainly through deubiquitinase-independent mechanisms. Intriguingly, partial rescue of TCR alpha (but not CFTR delta F508) in the ERAD pathway is also observed using a catalytically dead USP19, suggesting that USP19 exerts a non-catalytic function for select substrates in multiple biological pathways. This recombinant protein contains amino acids a C-terminal 6-His tag.

References
  1. Hassink G.C., et al. (2009) EMBO Rep. 10: 755
  2. Iphöfer, A. et al. (2012) ChemBioChem 13: 1416
  3. Mei, Y. et al. (2011) J. Biol. Chem. 286: 35380
Long Name
Ubiquitin Specific Protease 19
Entrez Gene IDs
10869 (Human); 71472 (Mouse); 361190 (Rat)
Alternate Names
Deubiquitinating enzyme 19; EC 3.1.2.15; EC 3.4.19.12; KIAA0891; Ubiquitin Carboxyl-Terminal Hydrolase 19; ubiquitin specific peptidase 19; ubiquitin thioesterase 19; Ubiquitin Thiolesterase 19; Ubiquitin-specific-processing protease 19; USP19; Zinc finger MYND domain-containing protein 9; ZMYND9; ZMYND9ubiquitin specific protease 19

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