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Recombinant Human His6-USP48 Protein, CF

Catalog #: E-614
1 Citation Datasheet / COA / SDS

Discontinued Product

E-614 has been discontinued.
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Recombinant Human His6-USP48 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human USP48 is a Ubiquitin-specific deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial USP48 concentration of 200-500 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP48 protein
Accession #
Q86UV5
Predicted Molecular Mass
120 kDa

Product Datasheets

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E-614

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-614

Formulation X mg/ml (X μM) in 50 mM HEPES pH7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USP48

USP48 is a deubiquitinating enzyme (DUB) of the C19 peptidase family.  Human USP48 has a predicted molecular weight of 119 kDa and is 93% and 94% identical to mouse and rat orthologues, respectively.  USP48 has been reported to trim K48-linked poly-Ubiquitin chains, an activity that is regulated by Casein Kinase 2 (CK2)-mediated phosphorylation of the DUB.  In conjunction with the COP9 signalosome (CSN), nuclear localized USP48 controls the proteasome-dependent turnover of activated NF-kappa B/RelA in the nucleus, thereby contributing to the control of immune responses.  USP48 has also been indicated in the regulation of blood pressure and sodium balance via interactions with the dopamine D3 receptor (D3R) and the Na+-H+ exchanger.  Finally, USP48 has been reported to stabilize Mdm2 in a manner that is not dependent on it deubiquitinating activity.  This recombinant protein contains an N-terminal 6-His tag.

References
  1. Armando I., et al. (2014) FASEB J.   28: 1422
  2. Cetkovska K., et al. (2017) Sci.Rep.   7: 43180
  3. Schweitzer K. & Naumann M. (2015) Biochim Biophys Acta.  1853: 453
Long Name
Ubiquitin Specific Protease 48
Entrez Gene IDs
84196 (Human); 170707 (Mouse); 362636 (Rat)
Alternate Names
Deubiquitinating enzyme 48; DKFZp762M1713; EC 3.1.2.15; EC 3.4.19.12; FLJ11328; FLJ20103; FLJ23054; FLJ23277; MGC132556; MGC14879; RAP1GA1; synUSP; ubiquitin carboxyl-terminal hydrolase 48; ubiquitin specific peptidase 48; ubiquitin specific protease 31; ubiquitin specific protease 48; ubiquitin thioesterase 48; Ubiquitin thiolesterase 48; Ubiquitin-specific-processing protease 48; USP31; USP48

Citation for Recombinant Human His6-USP48 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Accelerating inhibitor discovery for deubiquitinating enzymes
    Authors: WC Chan, X Liu, RS Magin, NM Girardi, SB Ficarro, W Hu, MI Tarazona G, CA Starnbach, A Felix, G Adelmant, AC Varca, B Hu, AS Bratt, E DaSilva, NJ Schauer, I Jaen Maiso, EK Dolen, AX Ayala, JA Marto, SJ Buhrlage
    Nature Communications, 2023-02-08;14(1):686.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay

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