Recombinant Human His6-USP48 Protein, CF

R&D Systems | Catalog # E-614

R&D Systems
Discontinued Product
E-614 has been discontinued. View all USP48 products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

Q86UV5

Applications

Bioactivity
View all USP48 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP48 protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

120 kDa

Activity

Recombinant Human USP48 is a Ubiquitin-specific deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial USP48 concentration of 200-500 nM.

Formulation, Preparation, and Storage

E-614
Formulation X mg/ml (X μM) in 50 mM HEPES pH7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP48

USP48 is a deubiquitinating enzyme (DUB) of the C19 peptidase family.  Human USP48 has a predicted molecular weight of 119 kDa and is 93% and 94% identical to mouse and rat orthologues, respectively.  USP48 has been reported to trim K48-linked poly-Ubiquitin chains, an activity that is regulated by Casein Kinase 2 (CK2)-mediated phosphorylation of the DUB.  In conjunction with the COP9 signalosome (CSN), nuclear localized USP48 controls the proteasome-dependent turnover of activated NF-kappa B/RelA in the nucleus, thereby contributing to the control of immune responses.  USP48 has also been indicated in the regulation of blood pressure and sodium balance via interactions with the dopamine D3 receptor (D3R) and the Na+-H+ exchanger.  Finally, USP48 has been reported to stabilize Mdm2 in a manner that is not dependent on it deubiquitinating activity.  This recombinant protein contains an N-terminal 6-His tag.

References

  1. Armando I., et al. (2014) FASEB J.   28: 1422
  2. Cetkovska K., et al. (2017) Sci.Rep.   7: 43180
  3. Schweitzer K. & Naumann M. (2015) Biochim Biophys Acta.  1853: 453

Long Name

Ubiquitin Specific Protease 48

Alternate Names

RAP1GA1, synUSP, USP31

Entrez Gene IDs

84196 (Human); 170707 (Mouse); 362636 (Rat)

Gene Symbol

USP48

UniProt

Q86UV5

Additional USP48 Products

Product Documents for Recombinant Human His6-USP48 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human His6-USP48 Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Recombinant Human His6-USP48 Protein, CF

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