Valosin-containing protein p97/p47 complex-interacting protein, or VCIP135, is a member of the OTU superfamily of Deubiquitinating Enzymes. It is 1222 amino acids (aa) in length with a predicted molecular weight of 134 kDa. Human VCIP135 shares 95% aa sequence identity with the mouse ortholog. In the cell, VCIP135 function requires membrane association and interaction with p97, both of which are inhibited by VCIP135 phosphorylation during mitosis. VCIP135 activity is necessary for VCP-mediated reassembly of Golgi stacks after mitosis. In vitro, VCIP135 efficiently hydrolyzes K11- and K48-linked Poly-ubiquitin chains.
Recombinant Human His6-VCIP135/VCPIP1 Protein, CF
R&D Systems | Catalog # E-590
Discontinued Product
E-590 has been discontinued.
View all VCIP135/VCPIP1 products.
Key Product Details
Source
Sf 21 (baculovirus)
Accession Number
Applications
Bioactivity
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Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VCIP135/VCPIP1 protein
Contains an C-terminal 6-His tag
Contains an C-terminal 6-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
135 kDa
Activity
Reaction conditions will need to be optimized for each specific application. We recommend an initial VCIP135 concentration of 100-500 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550 or U-555) or Poly-ubiquitin chain substrates.
Formulation, Preparation, and Storage
E-590
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: VCIP135/VCPIP1
References
- Zhang, X. et al. (2014) Cell Sci 127: 172-181
Long Name
Valosin-Containing Protein P97/P47 Complex-Interacting Protein p135
Alternate Names
DUBA3, VCPIP1
Gene Symbol
VCPIP1
UniProt
Additional VCIP135/VCPIP1 Products
Product Documents for Recombinant Human His6-VCIP135/VCPIP1 Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human His6-VCIP135/VCPIP1 Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human His6-VCIP135/VCPIP1 Protein, CF
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