Recombinant Human HPRG Protein, CF Summary
Val19-Lys525, with a C-terminal 10-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human histidine-rich glycoprotein (HPRG) is a multidomain, monomeric, secreted, 67-75 kDa member of the cystatin superfamily of molecules (1, 2). Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region (3). Five distinct domains are recognized in the mature molecule. There are two N-terminal cystatin-like modules (aa 19-254) and one His-Pro-rich region (aa 350-497) that is flanked by two Pro-rich segments (aa 276-321 and 498-525) (3, 4). The His-Pro-rich region contains 10 tandem repeats with an HHPHG motif, and the N- and C-termini are linked by a disulfide bond (3, 5, 6). Human HPRG is only 60% aa identical to mouse HPRG. There are multiple ligands for HPRG. These include small molecular weight molecules (metal ions; heme), hemostatic molecules (heparan sulfate; TSP; plasminogen), and immune system components (T cells; macrophages) (1, 5). About 50% of plasma plasminogen circulates bound to HPRG. Upon immobilization to cell surface tropomyosin in a Zn++‑dependent manner, it is converted to plasmin by tPA (7-9). HPRG also shows antiangiogenic activity on endothelial cells (10).
- Jones, A.L. et al. (2005) Immunol. Cell Biol. 83:106.
- Koide, T. and S. Odani (1987) FEBS Lett. 216:17.
- Koide, T. et al. (1986) Biochemistry 25:2220.
- SwissProt Accession # P04196.
- Borza, D-B. et al. (1996) Biochemistry 35:1925.
- Sorensen, C.B. et al. (1993) FEBS Lett. 328:285.
- Donate, F. et al. (2004) Cancer Res. 64:5812.
- Borza, D-B. and W.T. Morgan (1997) J. Biol. Chem. 272:5718.
- Guan, X. et al. (2004) Thromb. Haemost. 92:403.
- Juarez, J.C. et al. (2002) Cancer Res. 62:5344.
Citations for Recombinant Human HPRG Protein, CF
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Addiction to the IGF2-ID1-IGF2 circuit for maintenance of the breast cancer stem-like cells
Sample Types: Whole Cells
Insights into HER2 signaling from step-by-step optimization of anti-HER2 antibodies.
Authors: Fu W, Wang Y, Zhang Y, Xiong L, Takeda H, Ding L, Xu Q, He L, Tan W, Bethune A, Zhou L
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