Recombinant Human HR23A Tandem UBA (TUBE1) Agarose, CF

Catalog # Availability Size / Price Qty
AM-125-250
Product Details
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Recombinant Human HR23A Tandem UBA (TUBE1) Agarose, CF Summary

Human HR23A/Rad23A has two Ubiquitin-associated (UBA) motifs that can each bind Ubiquitin via a hydrophobic surface formed by residues located within the alpha 1 and alpha 3 helices of each UBA domain (1,2). Tandem-repeated Ubiquitin binding entities (TUBEs), which consist of multiple tandem Ubiquitin-binding UBA motifs, have been developed for the isolation and identification of ubiquitinated proteins (3). TUBEs show increased affinity for poly-Ubiquitin moieties compared to single UBA motifs (4). Additionally, TUBEs protect polyubiquitinated proteins from deubiquitylating enzymes, allowing for the detection of polyubiquitinated proteins at relatively low levels of abundance (5).

 

This affinity resin can be used for the enrichment, isolation and identification of K63-linked (preferentially) or K48-linked poly-Ub chains or ubiquitinated substrates that contain these linkages.

Specifications

Source
E. coli
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
  • 12 months from date of receipt, 2 to 8 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after opening.
Applications
Enzyme Activity
Species
Human

Product Datasheets

Background: HR23A/Rad23A TUBE1

References
  1. Chen, L. et al. (2001) EMBO Rep. 2:933.
  2. Wang, Q. et al. (2003) Biochemistry 42:13529.
  3. Hjerpe, R. et al. (2009) EMBO Rep. 10:1250.
  4. Hurley, J.H. et al. (2006) Biochem. J. 399:361.
  5. Lopitz-Otsoa, F. et al. (2010) Biochem. Soc. Trans. 38:40.
Long Name
RAD23 Homolog A
Entrez Gene IDs
5886 (Human); 19358 (Mouse); 361381 (Rat)
Alternate Names
HR23A; Rad23A

FAQs

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