Home / HR23A/Rad23A TUBE1 / Recombinant Human HR23A Tandem UBA (TUBE1) Agarose, CF

Recombinant Human HR23A Tandem UBA (TUBE1) Agarose, CF

Catalog #: AM-125 Datasheet / COA / SDS

Discontinued Product

AM-125 has been discontinued.
View all HR23A/Rad23A TUBE1 products.
Product Details
FAQs
Reviews
Summary Product Datasheets Background

Recombinant Human HR23A Tandem UBA (TUBE1) Agarose, CF Summary

Specifications

Source
E. coli
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
  • 12 months from date of receipt, 2 to 8 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after opening.
Applications
Enzyme Activity
Species
Human

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Background: HR23A/Rad23A TUBE1

Human HR23A/Rad23A has two Ubiquitin-associated (UBA) motifs that can each bind Ubiquitin via a hydrophobic surface formed by residues located within the alpha 1 and alpha 3 helices of each UBA domain (1,2). Tandem-repeated Ubiquitin binding entities (TUBEs), which consist of multiple tandem Ubiquitin-binding UBA motifs, have been developed for the isolation and identification of ubiquitinated proteins (3). TUBEs show increased affinity for poly-Ubiquitin moieties compared to single UBA motifs (4). Additionally, TUBEs protect polyubiquitinated proteins from deubiquitylating enzymes, allowing for the detection of polyubiquitinated proteins at relatively low levels of abundance (5).

 

This affinity resin can be used for the enrichment, isolation and identification of K63-linked (preferentially) or K48-linked poly-Ub chains or ubiquitinated substrates that contain these linkages.

References
  1. Chen, L. et al. (2001) EMBO Rep. 2:933.
  2. Wang, Q. et al. (2003) Biochemistry 42:13529.
  3. Hjerpe, R. et al. (2009) EMBO Rep. 10:1250.
  4. Hurley, J.H. et al. (2006) Biochem. J. 399:361.
  5. Lopitz-Otsoa, F. et al. (2010) Biochem. Soc. Trans. 38:40.
Long Name
RAD23 Homolog A
Alternate Names
hHR23A; HR23A

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human HR23A Tandem UBA (TUBE1) Agarose, CF

There are currently no reviews for this product. Be the first to review Recombinant Human HR23A Tandem UBA (TUBE1) Agarose, CF and earn rewards!

Have you used Recombinant Human HR23A Tandem UBA (TUBE1) Agarose, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review