Recombinant Human Hyaluronidase 2/HYAL2 His-tag Protein, CF

Catalog # Availability Size / Price Qty
Recombinant Human HYAL-2 His-tag Protein  (rhHYAL-2) Enzyme Activity Diagram
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Product Details
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Recombinant Human Hyaluronidase 2/HYAL2 His-tag Protein, CF Summary

Learn more about Fluorescent Glycan Labeling and Detection

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to digest Cy5-Labeled Hyaluronan (Low MW) to dp1-dp4.
The majority of Cy5-Labeled Hyaluronan (Low MW) (2 pmol) is digested by 0.25 μg of Recombinant Human HYAL-2, as measured under the described conditions.
Chinese Hamster Ovary cell line, CHO-derived human Hyaluronidase 2/HYAL2 protein
Met21-Gly447, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
50 kDa
53-58 kDa, under reducing conditions.

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

  • Assay Buffer: 50 mM MES, pH 5.0
  • Recombinant Human HYAL-2 (rhHYAL-2) (Catalog # 11012-GH)
  • Cy5-Labeled Hyaluronan (Low MW) (Cy5 LMW-HA) (Catalog # GL401)
  • 17% SDS-PAGE gel and SDS-PAGE Reagents
  • Reducing SDS-PAGE sample buffer
  • Fluorescent imager
  1. Dilute rhHYAL-2 to 25 ng/µL in Assay Buffer.
  2. Dilute Cy5 LMW-HA to 0.2 µM in Assay Buffer.
  3. Prepare reaction by combining 10 µL of diluted rhHYAL-2 and 10 µL of diluted Cy5 LMW-HA.
  4. Prepare a negative control by combining 10 µL of diluted Cy5 LMW-HA and 10 µL of Assay Buffer.
  5. Incubate reaction(s) and negative control at room temperature for 18 hours.
  6. Add 7 µL of the reducing SDS-PAGE sample buffer to each reaction and negative control.
  7. Load 13.5 µL each sample and control per well on a 17% SDS-PAGE gel and run SDS-PAGE until the dye front has migrated more than two thirds of the way down the gel.
  8. Acquire gel image with a fluorescent imager.
  9. Visually determine the digestion of Cy5 LMW-HA by rhHYAL-2 to dp 1-4 in each reaction lane.
Per Reaction:
  • rhHYAL-2: 0.25 µg
  • Cy5 LMW-HA: 2 pmol

Scientific Data

Enzyme Activity View Larger

rhHYAL-2 recognizes repeating subunits of -GlcA-beta 1,­3-GlcNAc-beta 1,4- and hydrolyzes high molecular weight hyaluronic acid to intermediate and smaller sized products. 

SDS-PAGE View Larger

1 μg/lane of rhHYAL-2 (Catalog # 11012-GH) was resolved with SDS-PAGE under reducing (R) conditions and visualized by Silver Staining, showing a band at 55 kDa.

Gel Supershift Assay View Larger

Lane 1 contained substrate Cy5-labeled LMW-HA GL401. In the presence of rhHYAL-2, the Hyaluronic Acid was modified to smaller molecular weight products with increasing amounts of rhHYAL-2 from left to right and a mobility shift was observed.

Reconstitution Calculator

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Background: Hyaluronidase 2/HYAL2

Human hyaluronidases (HYALs) are a group of five endo­-beta -­N­-acetyl­hexosaminidases that include HYAL1, HYAL2, HYAL3, HYAL4, and SPAM1 (PH20) (1, 2). While HYAL1, HYAL2 and SPAM1 are mainly active on hyaluronan (3, 4, 5), HYAL4 is active on both hyaluronan (6) and chondroitin sulfate (type C and D in particular) (7). Hyaluronan and chondroitin sulfate are abundant extracellular matrix components that have numerous biological functions (8). The backbone of chondroitin sulfate composed of repeating units of ­4-GlcA-beta 1,­3-GalNAc-beta 1­ most closely resembles to that of hyaluronan composed of repeating units of ­4-GlcA-beta 1,­3-GlcNAc-beta 1­. While HYAL1 is a strict lysosomal hyaluronidase and is active when pH ≤ 5.5 (9), HYAL2 is active in a narrow range of pH 5.0 to 5.5 (6) and is attached to cell membrane via GPI linker (10) where they may serve to hydrolyze high molecular weight hyaluronic acid of extracellular matrix to intermediate and small sized products.

  1. Csoka, A.B. et al. (2001) Matrix Biol. 20:499.
  2. Jedrzejas, M. J. and Stern, R. (2005) Proteins 61:227.
  3. Csoka, A.B. et al. (1999) Genomics 60:351.
  4. Lin, Y. et al. (1994) J. Cell Biol.125:1157.
  5. Lepperdinger, G. et al. (2001) Matrix Biol. 20:509.
  6. Wu, Z.L. and Ertelt, J.M. (2021) Glycobiology, cwab061,
  7. Kaneiwa, T. et al. (2010) Glycobiology 20:300.
  8. Iozzo, R.V. (1998) Annu. Rev. Biochem. 67:609.
  9. Hofinger, E.S.A. et al. (2007) Glycobiology 17:444.
  10. Rai, S.K. et al. (2001). Proc. Natl. Acad. Sci. U.S.A, 98:4443.
Long Name
Entrez Gene IDs
8692 (Human)
Alternate Names
EC; HYAL-2; Hyaluronidase 2; hyaluronoglucosaminidase 2; hyaluronoglucosaminidase-2; LUCA2; LUCA-2; LUCA2hyaluronidase-2; Lung carcinoma protein 2; lysosomal hyaluronidase; PH20 homolog; PH-20 homolog


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