Recombinant Human LAMP-2/CD107b Protein, CF

R&D Systems | Catalog # 6228-LM

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Human LAMP-2/CD107b Protein (6228-LM)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P13473

Applications

Bioactivity
View all LAMP-2/CD107b Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human LAMP-2/CD107b protein
Leu29-Phe375, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu29

Predicted Molecular Mass

39.7 kDa

SDS-PAGE

100-110 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LAMP2/CD107b is coated at 1 μg/mL, Recombinant Human Galectin‑3 (Catalog # 1154-GA) binds with an apparent KD <25 nM.

Formulation, Preparation, and Storage

6228-LM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 700 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: LAMP-2/CD107b

Lysosomal associated membrane protein 2 (LAMP2), also known as CD107b and LGP110, is an approximately 110 kDa transmembrane glycoprotein that is a major component of lysosomal membranes (1). Mature human LAMP2 consists of a 347 amino acid (aa) intralumenal domain, a 24 aa transmembrane segment, and a 35 aa cytoplasmic tail (2). Its lumenal domain is organized into two heavily N-glycosylated regions separated by a Ser/Pro-rich linker that carries a minor amount of O‑linked glycosylation (2, 3). Alternate splicing generates a human LAMP2 isoform (LAMP2B) with a substituted juxtamembrane lumenal region, transmembrane segment, and cytoplasmic tail (4). Within the lumenal domain, human LAMP2 shares approximately 64% aa sequence identity with mouse and rat LAMP2. LAMP2 itself is subject to lysosomal degradation following cleavage of its lumenal domain (5). It mediates the lysosomal uptake of the chaperone HSC73 in complex with cargo proteins and is required for the lysosomal destruction of autophagic vacuoles (6, 7). In cytotoxic T cells and mast cells, LAMP2 is expressed in the membranes of intracellular granules that contain effector molecules such as perforin, granzymes, eicosanoids, and histamine (8-10). Up‑regulated LAMP2 at the plasma membrane serves as an indicator of cell activation of CD8+ T cells, mast cells, monocytes, and platelets (9-12). LAMP2 is a native ligand for lectins Galectin-1 and Galectin-3 (13‑15).

References

  1. Eskelinen, E.-L. et al. (2003) Trends Cell Biol. 13:137.
  2. Fukuda, M. et al. (1988) J. Biol. Chem. 263:18920.
  3. Carlsson, S.R. et al. (1988) J. Biol. Chem. 263:18911.
  4. Konecki, D.S. et al. (1995) Biochem. Biophys. Res. Commun. 215:757.
  5. Cuervo, A.M. and J.F. Dice (2000) Traffic 1:570.
  6. Cuervo, A.M. and J.F. Dice (1996) Science 273:501.
  7. Tanaka, Y. et al. (1990) Nature 406:902.
  8. Peters, P.J. et al. (1991) J. Exp. Med. 173:1099.
  9. Betts, M.R. et al. (2003) J. Immunol. Meth. 281:65.
  10. Grutzkau, A. et al. (2004) Cytometry 61:62.
  11. Kannan, K. et al. (1996) Cell. Immunol. 171:10.
  12. Silverstein, R.L. and M. Febbraio (1992) Blood 80:1470.
  13. Skrincosky, D.M. et al. (1993) Cancer Res. 53:2667.
  14. Inohara, H. and Raz, A. (1994) Biochem. Biophys. Res. Commun. 201:1366.
  15. Ohannesian D.W. et al. (1994) Cancer Res. 54:5992.

Long Name

Lysosome-associated Membrane Glycoprotein 2

Alternate Names

CD107b, LAMP2, LAMPB, LGP110

Entrez Gene IDs

3920 (Human); 16784 (Mouse)

Gene Symbol

LAMP2

UniProt

P13473

Additional LAMP-2/CD107b Products

Product Documents for Recombinant Human LAMP-2/CD107b Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human LAMP-2/CD107b Protein, CF

For research use only

Citations for Recombinant Human LAMP-2/CD107b Protein, CF

Customer Reviews for Recombinant Human LAMP-2/CD107b Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human LAMP-2/CD107b Protein, CF and earn rewards!

Have you used Recombinant Human LAMP-2/CD107b Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes

Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail