Recombinant Human Galectin-3 Protein

Newer Version Available: 8259-GA-050
A New rh Galectin-3 is Now Available! Improved activity and purity; Mammalian cell expressed!
Formulations:
Catalog # Availability Size / Price Qty
1154-GA-050
Recombinant Human Galectin-3 Protein Bioactivity
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Product Details
Citations (6)
FAQs
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Recombinant Human Galectin-3 Protein Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED50 for this effect is 3‑10 µg/mL.
Source
E. coli-derived human Galectin-3 protein
Ala2-Ile250
Accession #
N-terminal Sequence
Analysis
Ala2
Predicted Molecular Mass
26 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1154-GA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Betamercaptoethanol with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.

1154-GA/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Betamercaptoethanol.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human Galectin-3 Protein Bioactivity View Larger

Recombinant Human Galectin-3 (Catalog # 1154-GA) agglutinates human red blood cells. The ED50 for this effectis 3-10 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-3

Human Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain (1, 2). Mature human Galectin-3 shares 78% and 79% amino acid (aa) sequence identity with mouse and rat Galectin-3, respectively. Human Galectin-3 is a 26 kDa protein that can be nuclear, cytoplasmic, or secreted (3, 4). Nuclear Galectin-3 can modulate gene expression, while cytosolic Galectin-3 can inhibit apoptosis and can participate in exocytosis, Caveolin-mediated endocytosis, and macrophage-mediated clearance of apoptotic cells (5-7). Extracellular Galectin-3 has been shown to form high-order oligomers that promote the crosslinking of cell surface oligosacchraides as well as integrin-dependent cell adhesion and apoptosis (8-11). Galectin-3 contributes to the innate immune response against Candida albicans and Streptococcus pneumoniae, and it can facilitate acute inflammatory responses via neutrophil activation and opsonization, macrophage recruitment, and mast cell activation (12-14). Galectin-3 can also contribute to chronic inflammation and fibrosis (15). It is implicated in neuroinflammatory disorders of the central nervous system, cardiac fibrosis, and heart failure, as well as tumor growth, progression, and metastasis (16-18).

 

References
  1. Robertson, M.W. et al. (1990) Biochemistry 29:8093.
  2. Elola, M.T. et al. (2007) Cell. Mol. Life Sci. 64:1679.
  3. Haudek, K.C. et al. (2010) Biochim. Biophys. Acta 1800:181.
  4. Hughes, R.C. (1999) Biochim. Biophys. Acta 1473:172.
  5. Krzeslak, A. and A. Lipinska (2004) Cell. Mol. Biol. Lett. 9:305.
  6. Dumic, J. et al. (2006) Biochim. Biophys. Acta 1760:616.
  7. Sano, H. et al. (2003) J. Clin. Invest. 112:389.
  8. Ahmad, N. et al. (2004) J. Biol. Chem. 279:10841.
  9. Friedrichs, J. et al. (2008) J. Biol. Chem. 283:32264.
  10. Wen, Y. et al. (2006) J. Cell. Biochem. 98:115.
  11. Zhuo, Y. et al. (2008) J. Biol. Chem. 283:22177.
  12. Henderson, N.C. and T. Sethi (2009) Immunol. Rev. 230:160.
  13. Kohatsu, L. et al. (2006) J. Immunol. 177:4718.
  14. Nieminen, J. et al. (2008) J. Immunol. 180:2466.
  15. Henderson, N.C. et al. (2008) Am. J. Pathol. 172:288.
  16. Newlaczyl, A.U. and L.G. Yu (2011) Cancer Lett. 313:123.
  17. Sherwi, N. et al. (2012) Future Cardiol. 8:885.
  18. Shin, T. (2013) Acta Histochem. 115:407.
Entrez Gene IDs
3958 (Human); 16854 (Mouse)
Alternate Names
AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3

Citations for Recombinant Human Galectin-3 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Galectin-3, a druggable vulnerability for KRAS-addicted cancers
    Authors: L Seguin, MF Camargo, HI Wettersten, S Kato, JS Desgrosell, T von Schals, KC Elliott, E Cosset, J Lesperance, SM Weis, DA Cheresh
    Cancer Discov, 2017;0(0):.
    Species: Human
    Sample Types: Purified Proteins
    Applications: Bioassay
  2. Secretion of IFN-? Associated with Galectin-9 Production by Pleural Fluid Cells from a Patient with Extrapulmonary Tuberculosis
    Authors: J Zhao, B Shiratori, H Chagan-Yas, M Matsumoto, T Niki, M Tanaka, Y Takahashi, O Usami, Y Ashino, T Hattori
    Int J Mol Sci, 2017;18(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Proteolytic activity of prostate-specific antigen (PSA) towards protein substrates and effect of peptides stimulating PSA activity.
    Authors: Mattsson J, Ravela S, Hekim C, Jonsson M, Malm J, Narvanen A, Stenman U, Koistinen H
    PLoS ONE, 2014;9(9):e107819.
    Species: Human
    Sample Types: Protein
    Applications: EnzAct
  4. Inhibition of galectin-3 reduces atherosclerosis in apolipoprotein E-deficient mice.
    Authors: Mackinnon, Alison C, Liu, Xiaojun, Hadoke, Patrick, Miller, Mark R, Newby, David E, Sethi, Tariq
    Glycobiology, 2013;23(6):654-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Binding of Toxoplasma gondii glycosylphosphatidylinositols to galectin-3 is required for their recognition by macrophages.
    Authors: Debierre-Grockiego F, Niehus S, Coddeville B, Elass E, Poirier F, Weingart R, Schmidt RR, Mazurier J, Guerardel Y, Schwarz RT
    J. Biol. Chem., 2010;285(43):32744-50.
    Species: Bacteria
    Sample Types: Complex Sample Type
    Applications: Surface Plasmon Resonance
  6. Galectin-3 is an amplifier of inflammation in atherosclerotic plaque progression through macrophage activation and monocyte chemoattraction.
    Authors: Papaspyridonos M, McNeill E, de Bono JP, Smith A, Burnand KG, Channon KM, Greaves DR
    Arterioscler. Thromb. Vasc. Biol., 2007;28(3):433-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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