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Recombinant Human MYSM1 Protein, CF

Catalog #: E-598 Datasheet / COA / SDS

Discontinued Product

E-598 has been discontinued.
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Recombinant Human MYSM1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human MYSM1 concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human MYSM1 protein
Accession #
Q5VVJ2
Predicted Molecular Mass
95 kDa

Product Datasheets

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E-598

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-598

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MYSM1

Histone H2A deubiquitinase MYSM1 is a specialized metalloprotease with a predicted molecular weight of 95 kDa. MYSM1 is a member of the peptidase M67A family and the human protein shares 79% amino acid sequence identity with its mouse ortholog. MYSM1 has been reported to function within a large chromatin remodeling complex, containing itself, PCAF, RBM10, TRIP5, and possibly other proteins. Biologically speaking, MYSM1 plays roles in hematopoiesis and lymphocyte differentiation, stem cell maintenance, and innate immunity.

References
  1. Belle J.I., et al. (2016) Cell Death Differ. 2016 doi: 10.1038/cdd.2015.140.
  2. Le Guen T., et al. (2015) J Allergy Clin. Immunol. 136: 1619
  3. Panda S, Nilsson J.A., Gekara N.O. (2015) Immunity 43: 647
  4. Zhu P., et al. (2007) Mol. Cell 27: 609
Long Name
Myb-Like, SWIRM And MPN Domains 1
Entrez Gene IDs
114803 (Human); 320713 (Mouse); 298247 (Rat)
Alternate Names
2A-DUB; DKFZp779J1554; DKFZp779J1721,2A-DUB; Histone H2A Deubiquitinase MYSM1; KIAA1915; Myb-like, SWIRM and MPN domains 1,2ADUB; MYSM1; RP4-592A1.1; SWIRM and MPN domain-containing protein 1

FAQs

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