Recombinant Human RNF114 Protein, CF

R&D Systems | Catalog # E3-304

R&D Systems
Discontinued Product
E3-304 has been discontinued. View all RNF114 products.

Key Product Details

Source

E. coli

Accession Number

Q9Y508
View all RNF114 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human RNF114 protein

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

26 kDa

SDS-PAGE

27 kDa

Scientific Data Images for Recombinant Human RNF114 Protein, CF

Recombinant Human RNF114 Protein SDS-PAGE

Recombinant Human RNF114 Protein SDS-PAGE

0.5 μg, 1 μg, and 2 μg per lane of Recombinant Human RNF114 (Catalog # E3-304) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 27 kDa.

Formulation, Preparation, and Storage

E3-304
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT
Shipping
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 48 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: RNF114

RING finger protein 114 (RNF114) is an E3 Ubiquitin Ligase containing both RING and C2HC type zinc finger domains.  RNF114 activates cell cycle progression and suppresses cellular senescence by ubiquitinating and destabilizing p21WAF1, p27KIP1 and p57KIP2, three members of cyclin-dependent kinase inhibitors.  RNF114 expression is elevated at the late G1 phase and is crucial for the G1-to-S phase transition.  Nimbolide, a terpenoid small molecule derived from Neem trees, interferes with RNF114-dependent ubiquitination of p21, resulting in its rapid stabilization.  Nimbolide has been shown to impair proliferation of breast cancer cells and is being used as a basis for development of targeted protein degradation.  This recombinant protein is untagged.

References

  1. Bodduluru L.N. et al. (2014) Tox. In Vitro  5: 1026
  2. Han J. et al. (2013) Cell Death & Diff.  20: 1055
  3. Spradlin J.N. et al. (2019) Nat. Chem. Biol.  15: 747

Long Name

Ring Finger Protein 114

Alternate Names

PSORS12, ZNF228, ZNF313

Entrez Gene IDs

55905 (Human)

Gene Symbol

RNF114

UniProt

Q9Y508

Additional RNF114 Products

Product Documents for Recombinant Human RNF114 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human RNF114 Protein, CF

For research use only

Related Research Areas

E3 Ubiquitin Ligases

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