Recombinant Human RNF114 Protein, CF

Discontinued Product

E3-304 has been discontinued.
View all RNF114 products.
Recombinant Human RNF114 Protein SDS-PAGE
1 Image
Product Details

Recombinant Human RNF114 Protein, CF Summary

Product Specifications

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
E. coli-derived human RNF114 protein
Accession #
Predicted Molecular Mass
26 kDa
27 kDa

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 48 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Data Image

SDS-PAGE Recombinant Human RNF114 Protein SDS-PAGE View Larger

0.5 μg, 1 μg, and 2 μg per lane of Recombinant Human RNF114 (Catalog # E3-304) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 27 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: RNF114

RING finger protein 114 (RNF114) is an E3 Ubiquitin Ligase containing both RING and C2HC type zinc finger domains.  RNF114 activates cell cycle progression and suppresses cellular senescence by ubiquitinating and destabilizing p21WAF1, p27KIP1 and p57KIP2, three members of cyclin-dependent kinase inhibitors.  RNF114 expression is elevated at the late G1 phase and is crucial for the G1-to-S phase transition.  Nimbolide, a terpenoid small molecule derived from Neem trees, interferes with RNF114-dependent ubiquitination of p21, resulting in its rapid stabilization.  Nimbolide has been shown to impair proliferation of breast cancer cells and is being used as a basis for development of targeted protein degradation.  This recombinant protein is untagged.

  1. Bodduluru L.N. et al. (2014) Tox. In Vitro  5: 1026
  2. Han J. et al. (2013) Cell Death & Diff.  20: 1055
  3. Spradlin J.N. et al. (2019) Nat. Chem. Biol.  15: 747
Long Name
Ring Finger Protein 114
Entrez Gene IDs
55905 (Human)
Alternate Names
PSORS12; RNF114; ZNF228; ZNF313


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