Recombinant Human RNF114 Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
0.5 μg, 1 μg, and 2 μg per lane of Recombinant Human RNF114 (Catalog # E3-304) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 27 kDa.
RING finger protein 114 (RNF114) is an E3 Ubiquitin Ligase containing both RING and C2HC type zinc finger domains. RNF114 activates cell cycle progression and suppresses cellular senescence by ubiquitinating and destabilizing p21WAF1, p27KIP1 and p57KIP2, three members of cyclin-dependent kinase inhibitors. RNF114 expression is elevated at the late G1 phase and is crucial for the G1-to-S phase transition. Nimbolide, a terpenoid small molecule derived from Neem trees, interferes with RNF114-dependent ubiquitination of p21, resulting in its rapid stabilization. Nimbolide has been shown to impair proliferation of breast cancer cells and is being used as a basis for development of targeted protein degradation. This recombinant protein is untagged.
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