Recombinant Human Semaphorin 3A Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1250-S3-025
Recombinant Human Semaphorin 3A Fc Chimera Protein Bioactivity
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Product Details
Citations (32)
FAQs
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Reviews (2)

Recombinant Human Semaphorin 3A Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) is coated at 2 µg/mL, Recombinant Human Semaphorin 3A Fc Chimera (Catalog # 1250-S3) binds with an ED50 = 50-300 ng/mL. Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 20-60 ng/mL of Recombinant Human Semaphorin 3A Fc Chimera causes >50% growth cone collapse in the presence 10 ng/mL Recombinant Human beta ‑NGF (Catalog # 256-GF).
Source
Mouse myeloma cell line, NS0-derived human Semaphorin 3A protein
Met-6-His tag Human Semaphorin 3A
Lys26-Val771
(Arg552Ala & Arg555Ala)
Accession # Q14563
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met & Ser770
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
113.5 kDa (monomer)
SDS-PAGE
120 kDa, 90 kDa and 37 kDa, reducing conditions

Product Datasheets

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1250-S3

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1250-S3

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Tween® 20.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human Semaphorin 3A Fc Chimera Protein Bioactivity View Larger

Recombinant Human Semaphorin 3A Fc Chimera binds to Recombinant Rat Neuropilin-1 Fc Chimera in an ELISA Binding Assay. When Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) is coated at 2 µg/ml, Recombinant Human Semaphorin 3A Fc Chimera (Catalog # 1250-S3) binds with an ED50 = 50-300 ng/mL.

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Background: Semaphorin 3A

The Semaphorins constitute a large family of secreted, GPI-anchored and transmembrane cell signaling molecules. Depending on their domain organization and species origin, these proteins can be classified into eight groups. To date, at least 19 vertebrate Semaphorins belonging to five groups (class 3 through 7) have been identified. All Semaphorins contain a conserved, 500 amino acid (aa) Sema domain at the amino terminus. Semaphorins are best known for their roles in axon guidance during neuronal development. Semaphorins are also expressed in non-neuronal tissues and are involved in angiogenesis, hematopoiesis, organogenesis, and the regulation of immune functions (1, 2).

Class 3 Semaphorins (Sema3) are secreted proteins containing a Sema domain, an immunoglobulin c2‑like domain and a basic domain near the carboxyl tail. Sema3A (also referred to as SemaIII, SemD and Collapsin) cDNA predicts a 771 aa precursor protein with a putative 25 aa signal peptide (1-3). Bioactive Sema3A is a disulfide-linked dimer (4). The bioactivity is increased after proteolytic processing by a furin-like endoprotease near the carboxy-terminus (1). The functional receptor complex for Sema3 is composed of two distinct transmembrane proteins: Neuropilin-1 (Npn-1) and Plexin-A. Npn-1 binds directly to Sema3A with high-affinity and confers specificity. Plexin-A interacts with Npn-1 to increase the affinity of the complex for Sema3A and serves as the signaling subunit in the receptor complex (1, 2, 5).

References
  1. Nakamura, F. et al. (2000) J Neurobiol. 44:219.
  2. Goshima, Y. et al. (2002) J. Clin. Invest. 109:993.
  3. Kolodkin, A.L. et al. (1993) Cell 75:1389.
  4. Koppel, A.M. et al. (1998) J. Biol. Chem. 273:15708.
  5. Yu, T.W. et al. (2001) Nature Neurosci. Supplement 4:1169.
  6. Luo Y. et al. (1993) Cell 75:217.
Entrez Gene IDs
10371 (Human); 20346 (Mouse); 29751 (Rat); 100620398 (Porcine); 102126862 (Cynomolgus Monkey)
Alternate Names
(semaphorin) 3A; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema III; SEMA1; Sema3A; SEMAD; SEMAIII; SEMAL; Semaphorin 3A; semaphorin D; Semaphorin III; semaphorin L; semaphorin-3A; SemD

Citations for Recombinant Human Semaphorin 3A Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. PlexinA4-Semaphorin3A-mediated crosstalk between main cortical interneuron classes is required for superficial interneuron lamination
    Authors: G Limoni, S Murthy, D Jabaudon, A Dayer, M Niquille
    Cell Reports, 2021;34(4):108644.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. A Potential Role of Semaphorin 3A during Orthodontic Tooth Movement
    Authors: S ?en, CJ Lux, R Erber
    International Journal of Molecular Sciences, 2021;22(15):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Vinaxanthone inhibits Semaphorin3A induced axonal growth cone collapse in embryonic neurons but fails to block its growth promoting effects on adult neurons
    Authors: E Ivakhnitsk, MR Chin, D Siegel, VH Guaiquil
    Scientific Reports, 2021;11(1):13019.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Reconsideration of the Semaphorin-3A Binding Motif Found in Chondroitin Sulfate Using Galnac4s-6st-Knockout Mice
    Authors: S Nadanaka, S Miyata, B Yaqiang, JI Tamura, O Habuchi, H Kitagawa
    Biomolecules, 2020;10(11):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  5. Semaphorin 3A mediated brain tumor stem cell proliferation and invasion in EGFRviii mutant gliomas
    Authors: DMO Higgins, M Caliva, M Schroeder, B Carlson, PS Upadhyayul, BD Milligan, SH Cheshier, IL Weissman, JN Sarkaria, FB Meyer, JR Henley
    BMC Cancer, 2020;20(1):1213.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1
    Authors: M Leclerc, E Voilin, G Gros, S Corgnac, V de Montpré, P Validire, G Bismuth, F Mami-Choua
    Nat Commun, 2019;10(1):3345.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. STIM1 is required for remodelling of the endoplasmic reticulum and microtubule cytoskeleton in steering growth cones
    Authors: M Pavez, AC Thompson, HJ Arnott, CB Mitchell, I D'Atri, EK Don, JK Chilton, EK Scott, JY Lin, KM Young, RJ Gasperini, L Foa
    J. Neurosci., 2019;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. B7-H4 Modulates Regulatory CD4+ T Cell Induction and Function via Ligation of a Semaphorin 3a/Plexin A4/Neuropilin-1 Complex
    Authors: JR Podojil, MY Chiang, I Ifergan, R Copeland, LN Liu, S Maloveste, S Langermann, D Liebenson, R Balabanov, H Chi, L Chen, DAA Vignali, SD Miller
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Class 3 semaphorins modulate the invasive capacity of rheumatoid arthritis fibroblast-like synoviocytes
    Authors: MW Tang, B Malvar Fer, SP Newsom, JD van Buul, TRDJ Radstake, DL Baeten, PP Tak, KA Reedquist, S García
    Rheumatology (Oxford), 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Stimulation
  10. Matrilysin/MMP-7 Cleavage of Perlecan/HSPG2 Complexed with Semaphorin 3A Supports FAK-Mediated Stromal Invasion by Prostate Cancer Cells
    Authors: BJ Grindel, JR Martinez, TV Tellman, DA Harrington, H Zafar, L Nakhleh, LW Chung, MC Farach-Car
    Sci Rep, 2018;8(1):7262.
    Applications: Bioassay
  11. Bidirectional regulation of bone formation by exogenous and osteosarcoma-derived Sema3A
    Authors: D de Ridder, S Marino, RT Bishop, N Renema, C Chenu, D Heymann, AI Idris
    Sci Rep, 2018;8(1):6877.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Semaphorin3A induces nerve regeneration in the adult cornea-a switch from its repulsive role in development
    Authors: M Zhang, Q Zhou, Y Luo, T Nguyen, MI Rosenblatt, VH Guaiquil
    PLoS ONE, 2018;13(1):e0191962.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Semaphorin 3A Increases FAK Phosphorylation at Focal Adhesions to Modulate MDA-MB-231 Cell Migration and Spreading on Different Substratum Concentrations
    Authors: S Gehler, FV Compere, AM Miller
    Int J Breast Cancer, 2017;2017(0):9619734.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. SEMA3A partially reverses VEGF effects through binding to neuropilin-1
    Authors: B Palodetto, A da Silva S, M Rodrigues, F Adolfo Cor, F Marconi Ro, F Soares Nie, K Priscila V, A Leda Figue, P Melo Campo, P Favaro, S Teresinha
    Stem Cell Res, 2017;22(0):70-78.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. MicroRNA-143 and -145 modulate the phenotype of synovial fibroblasts in rheumatoid arthritis
    Authors: BK Hong, S You, SA Yoo, D Park, D Hwang, CS Cho, WU Kim
    Exp. Mol. Med., 2017;49(8):e363.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells
    Authors: T Yokoi, T Tanaka, E Matsuzaka, F Tamalu, SI Watanabe, S Nishina, N Azuma
    Sci Rep, 2017;7(1):16757.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Brain endothelial cells control fertility through ovarian-steroid-dependent release of semaphorin 3A.
    Authors: Giacobini P, Parkash J, Campagne C, Messina A, Casoni F, Vanacker C, Langlet F, Hobo B, Cagnoni G, Gallet S, Hanchate N, Mazur D, Taniguchi M, Mazzone M, Verhaagen J, Ciofi P, Bouret S, Tamagnone L, Prevot V
    PLoS Biol, 2014;12(3):e1001808.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  18. Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells.
    Authors: Wada, Naohisa, Maeda, Hidefumi, Hasegawa, Daigaku, Gronthos, Stan, Bartold, Peter Ma, Menicanin, Danijela, Fujii, Shinsuke, Yoshida, Shinichi, Tomokiyo, Atsushi, Monnouchi, Satoshi, Akamine, Akifumi
    Stem Cells Dev, 2014;23(18):2225-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions.
    Authors: van Gils, Janine M, Ramkhelawon, Bhama, Fernandes, Luciana, Stewart, Merran C, Guo, Liang, Seibert, Tara, Menezes, Gustavo, Cara, Denise C, Chow, Camille, Kinane, T Bernar, Fisher, Edward A, Balcells, Mercedes, Alvarez-Leite, Jacqueli, Lacy-Hulbert, Adam, Moore, Kathryn
    Arterioscler Thromb Vasc Biol, 2013;33(5):911-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Semaphorin 3A suppresses tumor growth and metastasis in mice melanoma model.
    Authors: Chakraborty G, Kumar S, Mishra R
    PLoS ONE, 2012;7(3):e33633.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Measles virus modulates dendritic cell/T-cell communication at the level of plexinA1/neuropilin-1 recruitment and activity.
    Authors: Tran-Van H, Avota E, Bortlein C, Mueller N, Schneider-Schaulies S
    Eur. J. Immunol., 2011;41(1):151-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Syntaxin 1B suppresses macropinocytosis and semaphorin 3A-induced growth cone collapse.
    Authors: Kabayama H, Takeuchi M, Taniguchi M
    J. Neurosci., 2011;31(20):7357-64.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Specific association of increased vascular endothelial growth factor expression and its receptors with macrophage differentiation of HL-60 leukemia cells.
    Authors: Ohsaka A, Hirota-Komatsu S, Shibata M, Ezaki J, Shinohara F, Yoshida T
    Biochem. Biophys. Res. Commun., 2008;368(3):543-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. VEGF promotes vascular sympathetic innervation.
    Authors: Marko SB, Damon DH
    Am. J. Physiol. Heart Circ. Physiol., 2008;294(6):H2646-52.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Mitochondrial membrane potential in axons increases with local nerve growth factor or semaphorin signaling.
    Authors: Verburg J, Hollenbeck PJ
    J. Neurosci., 2008;28(33):8306-15.
    Species: Chicken
    Sample Types: Cell Lysates
    Applications: Bioassay
  26. Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice.
    Authors: Zacchigna S, Pattarini L, Zentilin L, Moimas S, Carrer A, Sinigaglia M, Arsic N, Tafuro S, Sinagra G, Giacca M
    J. Clin. Invest., 2008;118(6):2062-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Semaphorin 3A suppresses VEGF-mediated angiogenesis yet acts as a vascular permeability factor.
    Authors: Acevedo LM, Barillas S, Weis SM, Gothert JR, Cheresh DA
    Blood, 2008;111(5):2674-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A.
    Authors: Narazaki M, Segarra M, Tosato G
    Blood, 2008;111(8):4126-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. The original Pathologische Anatomie Leiden-Endothelium monoclonal antibody recognizes a vascular endothelial growth factor binding site within neuropilin-1.
    Authors: Jaalouk DE, Ozawa MG, Sun J, Lahdenranta J, Schlingemann RO, Pasqualini R, Arap W
    Cancer Res., 2007;67(20):9623-9.
    Species: N/A
    Sample Types: Virus
    Applications: Bioassay
  30. RhoA-kinase coordinates F-actin organization and myosin II activity during semaphorin-3A-induced axon retraction.
    Authors: Gallo G
    J. Cell. Sci., 2006;119(0):3413-23.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Ligand-induced internalization selects use of common receptor neuropilin-1 by VEGF165 and semaphorin3A.
    Authors: Narazaki M, Tosato G
    Blood, 2006;107(10):3892-901.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  32. Nerve growth factor, glial cell line-derived neurotrophic factor and neurturin prevent semaphorin 3A-mediated growth cone collapse in adult sensory neurons.
    Authors: Wanigasekara Y, Keast JR
    Neuroscience, 2006;142(2):369-79.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Is the Human IgG Fc on this protein, Catalog # 1250-S3, native?

    • The Fc tag on Catalog # 1250-S3 is a recombinant protein. It is Human IgG1 (Pro100-Lys330) Accession # P01857. It is similar to Catalog  # 110-HG.

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Reviews for Recombinant Human Semaphorin 3A Fc Chimera Protein, CF

Average Rating: 5 (Based on 2 Reviews)

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Recombinant Human Semaphorin 3A Fc Chimera Protein, CF
By Anonymous on 05/01/2017
Application: CellProlif
Reason for Rating: The proteins work as advertised. It induces cell morphological changes as well as inhibits cell growth.

Recombinant Human Semaphorin 3A Fc Chimera Protein, CF
By Anonymous on 06/08/2016
Application: In vitro bioactivity in cell culture