Recombinant Human Semaphorin 3E Protein, CF

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3239-S3B-025
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Semaphorin 3E Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al. (2010) Circ. Res. 106:391. The ED50 for this effect is 0.04-0.24 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin D1 (Catalog # 4160-PD) is coated at 5 μg/mL, Recombinant Human Semaphorin 3E binds with an apparent Kd <0.4 nM.

Source
Mouse myeloma cell line, NS0-derived human Semaphorin 3E protein
His24-Ser775 (Arg557Ala and Arg560Ala), with an N-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
His
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
88 kDa
SDS-PAGE
85-100 kDa, 66 kDa and 25 kDa, reducing conditions

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3239-S3B

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3239-S3B

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and Tween® 20 with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Semaphorin 3E

Semaphorin 3E (Sema3E), previously known as SemaH, is one of six Class 3 (secreted) semaphorins which function in axon guidance and/or vascular tip cell guidance during development (1). Sema3E contains a seven-blade beta -propeller sema domain, a cysteine-knot PSI domain, an Ig-like domain, and a basic region. Dimerization and cleavage within the basic region are required for the repulsing activity of class 3 semaphorins (2). Sema3E can also be cleaved at a furin consensus sequence C-terminal to the sema domain, resulting in a 61 kDa form that does not dimerize and is highly expressed in tumor cell lines with metastatic potential (3, 4). Mature human Sema3E shares 90% aa sequence identity with mouse and rat Sema3E. Alternative splicing generates a short isoform that lacks the signal peptide and the N-terminal 35 residues of the mature protein. Sema3E signaling is transduced by Plexin D1 which may also be associated with Neuropilin 1 and/or VEGF R2 (2, 5, 6). Its interaction with Plexin D1 inhibits axon migration in the neocortex and forebrain (6, 7), although it can attract axons that express both Plexin D1 and Neuropilin 1 (6). Sema3E promotes axonal growth (5), the development of glutamatergic synaptic specificity (8, 9), and the development of GnRH producing neurons (10). Genetic disruption of either Sema3E or Plexin D1 in mouse causes excessive and disorganized vascular growth and branching, indicating the importance of this ligand-receptor pair for vascular guidance (11, 12). In addition, Sema3E is up-regulated by inflammatory macrophages and damaged hepatocytes (13-15). It inhibits smooth muscle cell proliferation and migration in the asthmatic airway (16), promotes hepatic stellate cell activation and wound healing (15), and regulates the migration of developing thymocytes (17).

References
  1. Oh, W.J. and C. Gu (2013) Semin. Cell Dev. Biol. 24:156.
  2. Adams, R. H. et al. (1997) EMBO J. 16:6077.
  3. Christensen, C. et al. (2005) Cancer Res. 65:6167.
  4. Casazza, A. et al. (2010) J. Clin. Invest. 120:2684.
  5. Bellon, A. et al. (2010) Neuron 66:205.
  6. Chauvet, S. et al. (2007) Neuron 56:807.
  7. Bribian, A. et al. (2014) Nat. Commun. 5:4265.
  8. Ding, J.B. et al. (2011) Nat. Neurosci. 15:215.
  9. Pecho-Vrieseling, E. et al. (2009) Nature 459:842.
  10. Cariboni, A. et al. (2015) J. Clin. Invest. 125:2413.
  11. Gu, C. et al. (2005) Science 307:265.
  12. Gitler, A. D. et al. (2004) Developmental Cell 7:107.
  13. Wanschel, A. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33:886.
  14. Shimizu, I. et al. (2013) Cell Metab. 18:491.
  15. Yagai, T. et al. (2014) Am. J. Pathol. 184:2250.
  16. Movassagh, H. et al. (2014) J. Allergy Clin. Immunol. 133:560.
  17. Choi, Y.I. et al. (2014) Proc. Natl. Acad. Sci. USA 111:379.
Entrez Gene IDs
9723 (Human); 20349 (Mouse)
Alternate Names
(semaphorin) 3E; coll-5; KIAA0331M-SEMAH; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema3E; SEMAH; SEMAHM-SemaK; Semaphorin 3E; semaphorin-3E

Citations for Recombinant Human Semaphorin 3E Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Breast cancer bone metastases are attenuated in a Tgif1-deficient bone microenvironment
    Authors: MT Haider, H Saito, J Zarrer, K Uzhunnumpu, S Nagarajan, V Kari, M Horn-Gland, S Werner, E Hesse, H Taipaleenm
    Breast Cancer Res., 2020;22(1):34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Migration Bioassay
  2. Coronary vasculature patterning requires a novel endothelial ErbB2 holoreceptor
    Nat Commun, 2016;7(0):12038.
  3. Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages.
    Authors: Meda C, Molla F, De Pizzol M
    J. Immunol., 2012;188(8):4081-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation and potentiating RhoJ inactivation in endothelial cells.
    Authors: Kusuhara, Sentaro, Fukushima, Yoko, Fukuhara, Shigetom, Jakt, Lars Mar, Okada, Mitsuhir, Shimizu, Yuri, Hata, Masayuki, Nishida, Kohji, Negi, Akira, Hirashima, Masanori, Mochizuki, Naoki, Nishikawa, Shin-Ich, Uemura, Akiyoshi
    PLoS ONE, 2012;7(9):e45858.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Sema3E-PlexinD1 signaling selectively suppresses disoriented angiogenesis in ischemic retinopathy in mice.
    Authors: Fukushima Y, Okada M, Kataoka H
    J. Clin. Invest., 2011;121(5):1974-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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