Recombinant Human Semaphorin 3E Protein, CF

R&D Systems | Catalog # 3239-S3B

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Human Semaphorin 3E Protein (3239-S3B)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

O15041

Structure / Form

Disulfide-linked homodimer

Applications

Binding Activity, Bioactivity
View all Semaphorin 3E Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Semaphorin 3E protein
His24-Ser775 (Arg557Ala and Arg560Ala), with an N-terminal 10-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

88 kDa

SDS-PAGE

85-100 kDa, 66 kDa and 25 kDa, reducing conditions

Activity

Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al. (2010) Circ. Res. 106:391.
The ED50 for this effect is 0.04-0.24 μg/mL.

Measured by its binding ability in a functional ELISA.
When Recombinant Human Plexin D1 (Catalog # 4160-PD) is coated at 5 μg/mL, Recombinant Human Semaphorin 3E binds with an apparent Kd <0.4 nM.

Formulation, Preparation, and Storage

3239-S3B
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and Tween® 20 with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Semaphorin 3E

Semaphorin 3E (Sema3E), previously known as SemaH, is one of six Class 3 (secreted) semaphorins which function in axon guidance and/or vascular tip cell guidance during development (1). Sema3E contains a seven-blade beta -propeller sema domain, a cysteine-knot PSI domain, an Ig-like domain, and a basic region. Dimerization and cleavage within the basic region are required for the repulsing activity of class 3 semaphorins (2). Sema3E can also be cleaved at a furin consensus sequence C-terminal to the sema domain, resulting in a 61 kDa form that does not dimerize and is highly expressed in tumor cell lines with metastatic potential (3, 4). Mature human Sema3E shares 90% aa sequence identity with mouse and rat Sema3E. Alternative splicing generates a short isoform that lacks the signal peptide and the N-terminal 35 residues of the mature protein. Sema3E signaling is transduced by Plexin D1 which may also be associated with Neuropilin 1 and/or VEGF R2 (2, 5, 6). Its interaction with Plexin D1 inhibits axon migration in the neocortex and forebrain (6, 7), although it can attract axons that express both Plexin D1 and Neuropilin 1 (6). Sema3E promotes axonal growth (5), the development of glutamatergic synaptic specificity (8, 9), and the development of GnRH producing neurons (10). Genetic disruption of either Sema3E or Plexin D1 in mouse causes excessive and disorganized vascular growth and branching, indicating the importance of this ligand-receptor pair for vascular guidance (11, 12). In addition, Sema3E is up-regulated by inflammatory macrophages and damaged hepatocytes (13-15). It inhibits smooth muscle cell proliferation and migration in the asthmatic airway (16), promotes hepatic stellate cell activation and wound healing (15), and regulates the migration of developing thymocytes (17).

References

  1. Oh, W.J. and C. Gu (2013) Semin. Cell Dev. Biol. 24:156.
  2. Adams, R. H. et al. (1997) EMBO J. 16:6077.
  3. Christensen, C. et al. (2005) Cancer Res. 65:6167.
  4. Casazza, A. et al. (2010) J. Clin. Invest. 120:2684.
  5. Bellon, A. et al. (2010) Neuron 66:205.
  6. Chauvet, S. et al. (2007) Neuron 56:807.
  7. Bribian, A. et al. (2014) Nat. Commun. 5:4265.
  8. Ding, J.B. et al. (2011) Nat. Neurosci. 15:215.
  9. Pecho-Vrieseling, E. et al. (2009) Nature 459:842.
  10. Cariboni, A. et al. (2015) J. Clin. Invest. 125:2413.
  11. Gu, C. et al. (2005) Science 307:265.
  12. Gitler, A. D. et al. (2004) Developmental Cell 7:107.
  13. Wanschel, A. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33:886.
  14. Shimizu, I. et al. (2013) Cell Metab. 18:491.
  15. Yagai, T. et al. (2014) Am. J. Pathol. 184:2250.
  16. Movassagh, H. et al. (2014) J. Allergy Clin. Immunol. 133:560.
  17. Choi, Y.I. et al. (2014) Proc. Natl. Acad. Sci. USA 111:379.

Alternate Names

Sema3E, SEMAH

Entrez Gene IDs

9723 (Human); 20349 (Mouse)

Gene Symbol

SEMA3E

UniProt

O15041

Additional Semaphorin 3E Products

Product Documents for Recombinant Human Semaphorin 3E Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Semaphorin 3E Protein, CF

For research use only

Related Research Areas

Proteoglycans

Citations for Recombinant Human Semaphorin 3E Protein, CF

Customer Reviews for Recombinant Human Semaphorin 3E Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Semaphorin 3E Protein, CF and earn rewards!

Have you used Recombinant Human Semaphorin 3E Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes