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Recombinant Human Skp1 Protein, CF

Catalog #: E3-520 Datasheet / COA / SDS

Discontinued Product

E3-520 has been discontinued.
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Recombinant Human Skp1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical protein concentration for use in vitro will depend on experimental conditions.
Source
E. coli-derived human SKP1 protein
Accession #
P63208
Predicted Molecular Mass
19 kDa

Product Datasheets

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E3-520

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-520

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 6 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SKP1

SKP1 (S-phase kinase-associated protein 1) is an essential component of SCF (SKP1-CUL1-F-box protein) E3 Ubiquitin ligases that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.  SKP1 is the adapter protein that links Cullin-1 to various F-box proteins that serve as the substrate recognition components of SCF ligases.  This recombinant protein is untagged.

References
  1. Goldenberg S.J., et al.  (2004) Cell  119: 517
  2. Hristova V.A., et al. (2012) Mol. Cell  47: 331
  3. Wu G., et al.  (2003) Mol. Cell  11: 1445
Long Name
S-phase Kinase Associated Protein 1
Entrez Gene IDs
6500 (Human); 21402 (Mouse); 287280 (Rat)
Alternate Names
Cyclin-A/CDK2-associated protein p19; EMC19p19skp1; MGC34403; OCP2; OCP2OCP-2; OCP-IIS-phase kinase-associated protein 1A (p19A); Organ of Corti protein 2; Organ of Corti protein II; p19a; p19Acyclin A/CDK2-associated p19; p19Skp1; RNA polymerase II elongation factor-like protein OCP2; RNA polymerase II elongation factor-like protein; SKP1; SKP1A; SKP1Acyclin A/CDK2-associated protein p19; S-phase kinase-associated protein 1; TCEB1LSIII; Transcription elongation factor B

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