Recombinant Human SOCS-3 Protein, CF

R&D Systems | Catalog # E3-604

R&D Systems
Discontinued Product
E3-604 has been discontinued. View all SOCS-3 products.

Key Product Details

Source

E. coli

Accession Number

O14543

Applications

Bioactivity
View all SOCS-3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human SOCS-3 protein

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

25 kDa

Activity

Typical protein concentration for use in vitro will depend on experimental conditions.

Formulation, Preparation, and Storage

E3-604
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol,        1 mM DTT
Shipping
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: SOCS-3

The Suppressor of Cytokine Signaling (SOCS) protein family consists of 8 members including SOCS1-SOCS7, and CIS (cytokine-inducible SH2-containing protein).  SOCS proteins are a component of the negative feedback system that attenuates cytokine signaling pathways, acting principally by inhibiting the activation of JAK/STAT proteins.  Containing 95% identity with its rat and mouse orthologs, human SOCS3 is a 225 amino acid protein containing an N-terminal kinase inhibitory region, a central SH2 domain, and a C-terminal SOCS domain.  The SOCS domain mediates association of SOCS3 with the Elongin B/C adaptor complex of a CUL2/CUL5 ECS (Elongin BC-CUL2/5-SOCS-box) E3 Ubiquitin Ligase.  SOCS3 is the probable substrate recognition component of such ligases and may play a role in the ubiquitination of proteins including IL6 Receptor beta subunit (gp130), Insulin Receptor, Erythropoietin Receptor, leptin receptors and others.

References

  1. Kamura, T. et al. (2004) Genes Dev.  18: 3055
  2. Liu, S. et al. (2021) Front Cell Dev Biol   DOI: 10.3389/fcell.2021.629932
  3. Sasaki A., et al. (1999) Genes Cells   4:339
  4. Yamamoto K., et al. (2003)  Biochem. Biophys. Res. Commun.  310:1188

Long Name

Suppressor Of Cytokine Signaling 3

Alternate Names

ATOD4, CIS3, Cish3, SOCS3, SSI-3

Entrez Gene IDs

9021 (Human); 12702 (Mouse); 89829 (Rat)

Gene Symbol

SOCS3

UniProt

O14543

Additional SOCS-3 Products

Product Documents for Recombinant Human SOCS-3 Protein, CF

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Product Specific Notices for Recombinant Human SOCS-3 Protein, CF

For research use only

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