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Recombinant Human STAM-1 Protein, CF

Catalog #: E-550
2 Citations Datasheet / COA / SDS

Discontinued Product

E-550 has been discontinued.
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Recombinant Human STAM-1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Reaction conditions for stimulation of AMSH will need to be optimized for each specific application.  Binds AMSH protein with Kd = ~7 µM. In in vitro assays containing 200 nM AMSH, a ~10-fold increase in the rate of hydrolysis of K63-linked diubiquitin FRET substrate was achieved upon the addition of STAM. Half-maximal increase occurred at 0.4 μM STAM.
Source
E. coli-derived human STAM-1 protein
Contains an N-terminal Gly-Glu-Gly-Val-Arg-Thr sequence before Q92783 Met1.
Accession #
Q92783
Predicted Molecular Mass
60 kDa

Product Datasheets

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E-550

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-550

Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 2 mM DTT, 2 mM EDTA
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STAM-1

Signal Transducing Adaptor Molecule-1 (STAM-1) is a member the STAM family of adaptor molecules. This ubiquitously expressed protein is 540 amino acids (aa) in length with a predicted molecular weight of 59.18 kDa. Human STAM-1 shares 91% and 92% aa sequence identity with the rat and mouse orthologs, respectively. It contains multiple domains including a VHS domain (aa 16-143) and a Ubiquitin-interacting motif (aa 171-190), both of which bind ubiquitinated proteins, a SH3 domain (aa 210-269) that associates with the zinc metalloprotease STAMBP, and an ITAM domain (aa 370-387), which contains a Hrs-binding site and interacts with Janus kinases (Jaks) (1-6). STAM-1 is involved in downstream signaling stimulated by cytokines and growth factors. It is tyrosine phosphorylated by Jaks in response to a variety of cytokines including IL-2, IL-3, IL-4, IL-7, GM-CSF, EGF, and PDGF, and has been shown to induce DNA synthesis and MYC expression (1,2,5). Additionally, STAM-1 has been shown to regulate CXCR4 signaling and appears to be important for T cell development and survival (7,8). Besides its role in signal transduction, STAM-1 may also be important for the regulation of endocytic membrane trafficking. It associates with Hrs to form the endosomal sorting complex required for transport-0 (ESCRT-0) complex, which binds to ubiquitinated membrane proteins on early endosomes and directs them to the ESCRT-1 complex for lysosomal degradation (6, 9-11). STAM-1 has also been shown to interact with coat protein II complexes and function in trafficking vesicles from the endoplasmic reticulum to the Golgi apparatus (12). This full-length recombinant human protein has no intrinsic deubiquitinase activity but is useful in stimulating the in vitro activity of the JAMM-class deubiquitinase AMSH.

References
  1. Takeshita, T. et al. (1996) Biochem. Biophys. Res. Commun. 225:1035.
  2. Takeshita, T. et al. (1997) Immunity 6:449.
  3. Asao, H. et al. (1997) J. Biol. Chem. 272:32785.
  4. Tanaka, N. et al. (1999) J. Biol. Chem. 274:19129.
  5. Lohi, O. & V.P. Lehto (2001) FEBS Lett. 508:287.
  6. Mizuno, E. et al. (2003) Mol. Biol. Cell 14:3675.
  7. Yamada, M. et al. (2002) Mol. Cell. Biol. 22:8648.
  8. Malik, R. et al. (2012) J. Biol. Chem. 287:9013.
  9. Bache, K.G. et al. (2003) J. Biol. Chem. 278:12513.
  10. Mizuno, E. et al. (2004) J. Biochem. 135:385.
  11. Ren, X. & J.H. Hurley (2010) EMBO J. 29:1045.
  12. Rismanchi, N. et al. (2009) Traffic 10:201.
Long Name
Signal Transducing Adaptor Molecule 1
Entrez Gene IDs
8027 (Human); 20844 (Mouse); 498798 (Rat)
Alternate Names
DKFZp686J2352; HSE1 Homolog; signal transducing adapter molecule 1; signal transducing adaptor molecule (SH3 domain and ITAM motif) 1; STAM; STAM1; STAM-1; STAM1STAM-1

Citations for Recombinant Human STAM-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Structural and functional characterization of ubiquitin variant inhibitors for the JAMM-family deubiquitinases STAMBP and STAMBPL1
    Authors: Y Guo, Q Liu, E Mallette, C Caba, F Hou, J Fux, G LaPlante, A Dong, Q Zhang, H Zheng, Y Tong, W Zhang
    The Journal of Biological Chemistry, 2021-08-21;0(0):101107.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activity
    Authors: JS Bednash, N Weathingto, J Londino, M Rojas, DL Gulick, R Fort, S Han, AC McKelvey, BB Chen, RK Mallampall
    Nat Commun, 2017-05-11;8(0):15203.
    Species: Human
    Sample Types: Protein, Recombinant Protein
    Applications: Bioassay

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