Recombinant Human Tetra-Ub (K63-linked) Rhodamine 110, CF

R&D Systems | Catalog # UC-355

R&D Systems
Discontinued Product
UC-355 has been discontinued. View all Tetra-Ubiquitin products.

Key Product Details

Source

E. coli

Accession Number

P0CG47

Conjugate

Rhodamine 110

Applications

Bioactivity
View all Tetra-Ubiquitin Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Tetra-Ubiquitin protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

35 kDa

Activity

K63-linked tetra-Ubiquitin Rhodamine 110 is ideal for use in assays requiring fluorescent detection of deubiquitinase activity.  Optimal fluorescence at pH 8.0 is monitored with excitation and emission wavelengths of 485 nm and 535 nm, respectively.  Reaction conditions will need to optimized for each specific application.  We recommend an initial concentration of 0.1-1 μM.

Reviewed Applications

Read 1 review rated 5 using UC-355 in the following applications:

Formulation, Preparation, and Storage

UC-355
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.0, 50 mM NaCl
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Tetra-Ubiquitin

Linkage specific poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB's") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules. K63-linked tetra-Ubiquitin chains are manufactured using recombinant wild-type human Ubiquitin and linkage-specific enzymes. The use of purely enzymatic techniques avoids the potential for contaminating synthetic intermediates. This fluorogenic substrate is intended for use with deubiquitinating enzymes that are unable to use mono-Ubiquitin-Rh110 (Catalog # http://www.rndsystems.com/product_results.aspx?k=U-555">U-555) as a substrate, or enzymes with activity that is stimulated by poly-Ubiquitin chains. The substrate consists of a K63-linked tetra-Ubiquitin chain with a single rhodamine attached to the C-terminus of the proximal Ubiquitin.

Alternate Names

TetraUbiquitin, Ub4

Entrez Gene IDs

7314 (Human)

Gene Symbol

UBB

UniProt

P0CG47

Product Documents for Recombinant Human Tetra-Ub (K63-linked) Rhodamine 110, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Tetra-Ub (K63-linked) Rhodamine 110, CF

For research use only

Related Research Areas

NFkB Pathway

Citations for Recombinant Human Tetra-Ub (K63-linked) Rhodamine 110, CF

Customer Reviews for Recombinant Human Tetra-Ub (K63-linked) Rhodamine 110, CF (1)

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    Application: Enzymatic activity in vitro
    Verified Customer | Posted 07/02/2019
    Recombinant Human Tetra-Ub (K63-linked) Rhodamine 110, CF UC-355

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