Recombinant Human TGF-beta 1.2 Protein

  (1 citations)     
Datasheet / CoA / SDS
Product Details
Citations (1)
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  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.08 ng/mL.
  • Source
    Spodoptera frugiperda, Sf 21 (baculovirus)-derived human TGF-beta 1.2 protein
    Human TGF-beta 1
    (Ala279 - Ser390)
    Accession # P01137
    Human TGF-beta 2
    (Ala303 - Ser414)
    Accession # P61812
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala279 (TGF-beta 1) & Ala303 (TGF-beta 2)
  • Structure / Form
    Disulfide-linked heterodimer
  • Predicted Molecular Mass
    12.8 kDa (TGF-beta 1) & 12.7 kDa (TGF-beta 2)
Product Datasheets

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Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
304-B3
 
304-B3/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
 
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 10 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
 
Reconstitution Reconstitute at 10 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 1.2

Transforming Growth Factor Beta (TGF-beta ) is a stable, multifunctional polypeptide growth factor. While specific receptors for this protein have been found on almost all mammalian cell types thus far examined, the effect of the molecule varies depending on the cell type and growth conditions. Generally, TGF-beta is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodermal origin. It is now known that the originally described form of TGF-beta, now described as TGF-beta 1, is only one of a family of regulatory proteins consisting of a number of proteins distantly related to TGF-beta 1 (30 - 40% sequence homology) and a number of more closely related proteins (70 - 80% sequence homology) designated TGF-beta 2, TGF-beta 1.2, TGF-beta 3, TGF-beta 4, and TGF-beta 5. TGF-beta 1 has been found in the highest concentration in human platelets and mammalian bone, but is produced by many cell types in smaller amounts. TGF-beta 2 has been found in the highest concentration in porcine platelets and mammalian bone, but again is also produced by many types of cells. The heterodimer, TGF-beta 1.2, has so far been found only in small amounts in porcine platelets. TGF-beta 3 has been detected in human, porcine, and avian sources, mainly in cells of mesenchymal origin, suggesting a different role for this protein than for TGF-beta 1 or -beta 2. TGF-beta 4 has been detected in chick embryo chondrocytes, and its distribution in other types of cells is being investigated. TGF-beta 5 has been detected only in Xenopus embryos. TGF-beta 1, TGF-beta 2, and TGF-beta 1.2 appear to be largely equivalent in biological activity, although there appear to be differences in binding to certain types of receptors, and there are a few reports of differential responses to TGF-beta 1 and TGF-beta 2.

  • Long Name:
    Transforming Growth Factor beta 1.2
  • Alternate Names:
    TGFbeta 1.2; TGF-beta 1.2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Cutting edge: immune cells as sources and targets of the IL-10 family members?
    Authors: Wolk K, Kunz S, Asadullah K, Sabat R
    J. Immunol., 2002;168(11):5397-402.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

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