Recombinant Human UCH-L1/PGP9.5 Protein, CF

R&D Systems | Catalog # E-340

R&D Systems
Discontinued Product
E-340 has been discontinued. View all UCH-L1/PGP9.5 products.

Key Product Details

Source

E. coli

Accession Number

P09936

Applications

Enzyme Activity
View all UCH-L1/PGP9.5 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human UCH-L1/PGP9.5 protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

25 kDa

Activity

Recombinant Human UCH-L1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UCH-L1 concentration of 10-50 nM. A 15 minute pre-incubation with 10 mM DTT is recommended to achieve maximum activity.

Reviewed Applications

Read 1 review rated 5 using E-340 in the following applications:

Formulation, Preparation, and Storage

E-340
Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM EDTA, 2 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: UCH-L1/PGP9.5

Ubiquitin Carboxyl-terminal Esterase L1 (UCH-L1), also known as PGP 9.5, is a deubiquitinating enzyme with a predicted molecular weight of 25 kDa (1). The human protein shares 95% amino acid sequence identity with its mouse and rat orthologs. UCH-L1 is expressed abundantly in neurons, accounting for 1-2% of total soluble proteins in the brain (2). It localizes primarily to the cytoplasm, but a subpopulation has been shown to be transiently nuclear (3,4). UCH-L1 contains two catalytic residues, Cys90 and His161, which are required for isopeptide bond cleavage at the C-terminal glycine residue of Ubiquitin (5). The levels of free Ubiquitin appear to be partially regulated by UCH-L1 through the hydrolysis of small Ubiquitin chains and the stabilization of monomeric Ubiquitin (6). Mice lacking functional UCH-L1 show neuronal dysfunction and neurodegeneration, and mutations in this enzyme have been linked to Parkinson's disease, suggesting that it is important for proper central nervous system function (7,8). UCH-L1 also likely plays a complex role in cancer. It has been reported to function as an oncogene in lymphoma, colorectal cancer, and nonsmall cell lung carcinoma (9-11). In contrast, it is thought to function as a tumor suppressor protein in nasopharyngeal and breast cancers (12,13).

References

  1. Wilkinson, K.D. et al. (1989) Science 246:670.
  2. Solano, S.M. et al. (2000) Ann. Neurol. 47:201.
  3. Henderson, M.J. et al. (2010) J. Biol. Chem. 285:11314.
  4. Bheda, A. et al. (2010) Cell Cycle 9:980.
  5. Larsen, C.N. et al. (1996) Biochemistry 35:6735.
  6. Walters, B.J. et al. (2008) Mol. Cell. Neurosci. 39:539.
  7. Sakurai, M. et al. (2008) Eur. J. Neurosci. 27:691.
  8. Andersson, F.I. et al. (2011) J. Mol. Biol. 407:261.
  9. Hussain, S. et al. (2010) Leukemia 24:1641.
  10. Akishima-Fukasawa, Y. et al. (2010) Am. J. Clin. Pathol. 134:71.
  11. Kim, H.J. et al. (2009) Oncogene 28:117.
  12. Li, L. et al. (2010) Clin. Cancer Res. 16:2949.
  13. Xiang, T. et al. (2012) PLoS One 7:e29783.
 

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Entrez Gene IDs

7345 (Human); 22223 (Mouse)

Gene Symbol

UCHL1

UniProt

P09936 (Human)

Additional UCH-L1/PGP9.5 Products

Product Documents for Recombinant Human UCH-L1/PGP9.5 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human UCH-L1/PGP9.5 Protein, CF

For research use only

Citations for Recombinant Human UCH-L1/PGP9.5 Protein, CF

Customer Reviews for Recombinant Human UCH-L1/PGP9.5 Protein, CF (1)

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    Recombinant Human UCH-L1/PGP9.5 Protein, CF E-340

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