Recombinant Human UCH-L5/UCH37 Protein, CF
Recombinant Human UCH-L5/UCH37 Protein, CF Summary
Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human UCH-L5 concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 1 mM TCEP, 10% (v/v) Glycerol,|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases (known as the peptidase C12 family) that catalyze the hydrolysis of amides, esters and thioesters from the c-terminal end of Ubiquitin. Human UCH-L5 (also known as UCH37) is 329 amino acids long with a predicted weight of 38 kDa, and shares 97% sequence identity with its mouse ortholog. UCH-L5 is found associated with the 19S regulatory complex of the 26S proteasome, where it functions to cleave K48-linked poly-Ubiquitin chains from Ubiquitinated substrates. UCH-L5 is also found as a component of the INO80 complex, where its activity is regulated by a mechanism involving ADRM1.
Burgie S.E., et al. (2012) Proteins 80: 649
Yao T., et al. (2006) Nat. Cell Biol. 8: 994
- Yao T., et al. (2008) Mol. Cell 31: 909
Citation for Recombinant Human UCH-L5/UCH37 Protein, CF
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1 Citation: Showing 1 - 1
Activity-Based Probes for HECT E3 ubiquitin ligases
Authors: R Byrne, T Mund, J Licchesi
Sample Types: Recombinant Protein
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