Recombinant Human UCH-L5/UCH37 Protein, CF

Catalog # Availability Size / Price Qty
E-327-025
Product Details
Citations (1)
FAQs
Reviews

Recombinant Human UCH-L5/UCH37 Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human UCH-L5 concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)

Source
E. coli-derived human UCH-L5/UCH37 protein
Accession #
Predicted Molecular Mass
38 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-327

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 1 mM TCEP, 10% (v/v) Glycerol,
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: UCH-L5/UCH37

Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases (known as the peptidase C12 family) that catalyze the hydrolysis of amides, esters and thioesters from the c-terminal end of Ubiquitin. Human UCH-L5 (also known as UCH37) is 329 amino acids long with a predicted weight of 38 kDa, and shares 97% sequence identity with its mouse ortholog. UCH-L5 is found associated with the 19S regulatory complex of the 26S proteasome, where it functions to cleave K48-linked poly-Ubiquitin chains from Ubiquitinated substrates. UCH-L5 is also found as a component of the INO80 complex, where its activity is regulated by a mechanism involving ADRM1.

References
  1. Burgie S.E., et al. (2012) Proteins 80: 649
  2. Yao T., et al. (2006) Nat. Cell Biol. 8: 994
  3. Yao T., et al. (2008) Mol. Cell 31: 909
Long Name
Ubiquitin C-terminal Hydrolase L5
Entrez Gene IDs
51377 (Human); 56207 (Mouse); 360853 (Rat)
Alternate Names
CGI-70; EC 3.4.19.12; INO80 complex subunit R; INO80R; ubiquitin carboxyl-terminal esterase L5; ubiquitin carboxyl-terminal hydrolase isozyme L5; ubiquitin carboxyl-terminal hydrolase L5; Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5; UCH37; UCH37CGI-70; UCHL5; UCH-L5

Citation for Recombinant Human UCH-L5/UCH37 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Activity-Based Probes for HECT E3 ubiquitin ligases
    Authors: R Byrne, T Mund, J Licchesi
    Chembiochem, 2017;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human UCH-L5/UCH37 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human UCH-L5/UCH37 Protein, CF and earn rewards!

Have you used Recombinant Human UCH-L5/UCH37 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review