Recombinant Human UCH-L5/UCH37 Protein, CF

R&D Systems | Catalog # E-327

R&D Systems
Discontinued Product
E-327 has been discontinued. View all UCH-L5/UCH37 products.

Key Product Details

Source

E. coli

Accession Number

Q9Y5K5

Applications

Bioactivity
View all UCH-L5/UCH37 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human UCH-L5/UCH37 protein

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

38 kDa

Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human UCH-L5 concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)

Formulation, Preparation, and Storage

E-327
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 1 mM TCEP, 10% (v/v) Glycerol,
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: UCH-L5/UCH37

Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases (known as the peptidase C12 family) that catalyze the hydrolysis of amides, esters and thioesters from the c-terminal end of Ubiquitin. Human UCH-L5 (also known as UCH37) is 329 amino acids long with a predicted weight of 38 kDa, and shares 97% sequence identity with its mouse ortholog. UCH-L5 is found associated with the 19S regulatory complex of the 26S proteasome, where it functions to cleave K48-linked poly-Ubiquitin chains from Ubiquitinated substrates. UCH-L5 is also found as a component of the INO80 complex, where its activity is regulated by a mechanism involving ADRM1.

References

  1. Burgie S.E., et al. (2012) Proteins 80: 649
  2. Yao T., et al. (2006) Nat. Cell Biol. 8: 994
  3. Yao T., et al. (2008) Mol. Cell 31: 909

Long Name

Ubiquitin C-terminal Hydrolase L5

Alternate Names

CGI-70, INO80R, UCH37, UCHL5

Entrez Gene IDs

51377 (Human); 56207 (Mouse); 360853 (Rat)

Gene Symbol

UCHL5

UniProt

Q9Y5K5

Additional UCH-L5/UCH37 Products

Product Documents for Recombinant Human UCH-L5/UCH37 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human UCH-L5/UCH37 Protein, CF

For research use only

Citations for Recombinant Human UCH-L5/UCH37 Protein, CF

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