Recombinant Mouse Brevican Protein, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Supplemental Products

Recombinant Mouse Brevican Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA.

When Recombinant Mouse Brevican is coated at 3 μg/mL (100 μL/well), the concentration of biotinylated hyaluronan that produces 50% of the optimal binding response is found to be approximately 1-6 ng/mL.

Mouse myeloma cell line, NS0-derived mouse Brevican protein
Met1-Leu883, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
94.5 kDa
130-145 kDa, reducing conditions

Complete Your Experiment

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Brevican

Brevican, also called BEHAB, is a member of the the lectican family of proteoglycans that share a common domain structure (1). It is restricted to the CNS and associates with perineuronal nets and astrocytes (2 ‑ 5). Brevican contains an Ig-like V-set domain, two link domains, a Glu-rich region, a central region with glycosaminoglycan (GAG) modifications, an EGF-like domain, a C-type lectin domain, and a C-terminal Sushi/CRP-like domain (6). Brevican consists of a 145 kDa core protein with up to approximately 100 kDa of attached chondroitin sulfate but not heparan sulfate chains (3, 7). Alternate splicing generates a GPI-anchored isoform that lacks the EGF-like, lectin, and Sushi domains (4, 6). Brevican is cleaved by multiple proteases and exists in a number of distinct fragments (8, 9). An under-glycosylated form of Brevican accumulates in highly aggressive glioma, Alzheimer’s-like brain, and following brain injury (10 - 12). Levels of an ADAMTS-generated 55 kDa N-terminal fragment accumulate during remodeling after excitotoxic injury, and Brevican cleavage is required for its ability to promote glioma adhesion and motility (13 ‑ 15). In contrast, Brevican cleavage is inhibited in mouse brain with amyloid beta deposits (10). Mouse Brevican shares 80%, 81%, and 95% aa sequence identity with bovine, human, and rat Brevican, respectively. Within the Ig-like and two link domains, mouse Brevican shares 47% - 51% aa sequence identity with mouse Aggrecan, Neurocan, and Versican.

  1. Maeda, N. et al. (2010) Front. Biosci. 15:626.
  2. Jaworski, D.M. et al. (1994) J. Cell Biol. 125:495.
  3. Yamada, H. et al. (1997) J. Neurosci. 17:7784.
  4. Seidenbecher, C.I. et al. (2002) J. Neurochem. 83:738.
  5. Deepa, S.S. et al. (2006) J. Biol. Chem. 281:17789.
  6. Rauch, U. et al. (1997) Genomics 44:15.
  7. Seidenbecher, C.I. et al. (1995) J. Biol. Chem. 270:27206.
  8. Matthews, R.T. et al. (2000) J. Biol. Chem. 275:22695.
  9. Nakamura, H. et al. (2000) J. Biol. Chem. 275:38885.
  10. Ajmo, J.M. et al. (2010) J. Neurochem. 113:784.
  11. Viapiano, M.S. et al. (2005) Canc. Res. 65:6726.
  12. Viapiano, M.S. et al. (2003) J. Biol. Chem. 278:33239.
  13. Hu, B. et al. (2008) J. Biol. Chem. 283:24848.
  14. Mayer, J. et al. (2005) BMC Neurosci. 6:52.
  15. Yuan, W. et al. (2002) Neuroscience 114:1091.
Long Name
Brain Specific Proteoglycan in the Aggrecan Family
Entrez Gene IDs
63827 (Human); 12032 (Mouse); 25393 (Rat)
Alternate Names
ALPBRE; BCAN; BEHAB; BEHABbrevican core protein; Brain-enriched hyaluronan-binding protein; Brevican; Chondroitin sulfate proteoglycan 7; chondroitin sulfate proteoglycan BEHAB; chondroitin sulfate proteoglycan BEHAB/brevican; CSPG7; CSPG7brevican proteoglycan; MGC13038


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Mouse Brevican Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse Brevican Protein, CF and earn rewards!

Have you used Recombinant Mouse Brevican Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review